We narrowed to 25,057 results for: Nes

1. pcDNA-TAL-NC2

   Plasmid

   #43855

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

   Insert

   TALEN-FokI nuclease backbone
   

   Use

   Tags

   Flag and FokI nuclease

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-TAL-NC2

   Plasmid

   #43856

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

   Insert

   TALEN-FokI nuclease backbone
   

   Use

   Tags

   Flag and FokI nuclease

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. GRM1

   Plasmid

   #25353

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   GRM1-C254S:L28-G518 (GRM1 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   C254S. Contains amino acids L28-G518.

   Promoter

   Available Since

   July 29, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pSR-puro-Sec6

   Plasmid

   #31118

   Depositor

   Christopher Counter

   Article

   Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

   Insert

   Sec6 shRNA (EXOC3 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pTGC-U6.2

   Plasmid

   #112809

   Purpose

   PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Insert

   U6:2-gRNAcore
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pONL-N1_PTS2

   Plasmid

   #65677

   Purpose

   Expresses PTS2-ONL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 2
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR - C9orf114 sgRNA 1

   Plasmid

   #70655

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C9orf114
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. lentiCRISPR - C9orf114 sgRNA 2

   Plasmid

   #70654

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C9orf114
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Cerulean(159-238) in pcDNAI/Amp

   Plasmid

   #60898

   Purpose

   This is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   Cerulean(159-238)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains N164H substitution

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCNL-C1_H2B

    Plasmid

    #65703

    Purpose

    Expresses CNL-H2B in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    histone H2B
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMT-PT2A-Tdtomato-GFP-iRFP670

    Plasmid

    #111814

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Tdtomato
    GFP
    iRFP670
    

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST96/LBT-MCS

    Plasmid

    #108227

    Purpose

    Bacterial expression expression of protein with N-terminal lanthanide binding tag

    Depositor

    Didier Picard

    Article

    Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

    Insert

    LBT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, lacO

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPOR0SP0016

    Plasmid

    #117526

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K810A, K1003A, R1060A

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRRNA-G0-R20

    Plasmid

    #101818

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRRNA-G20-R0

    Plasmid

    #101807

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-R1ACR_653-EYFP

    Plasmid

    #111079

    Purpose

    Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin R1ACR_653
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSALECTNK-LGK/csTEM1

    Plasmid

    #81164

    Purpose

    pSALECT plasmid with levoglucosan kinase fused to TEM-1

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    Levoglucosan kinase
    

    Use

    Tags

    Codon swapped TEM-1 beta-lactamase (H26 to W290) …

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO HIS DNAJB3

    Plasmid

    #19552

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. ARL5:Q15-L176 (2H17)

    Plasmid

    #25527

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARL5:Q15-L176 (ARL5A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids Q15-L176

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. ARF17 pGreenII0229

    Plasmid

    #12070

    Depositor

    David Bartel

    Article

    Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

    Insert

    ARF17 (ARF17 Mustard Weed)
    

    Use

    Plant transformation via agrobacterium

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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