We narrowed to 57,620 results for: {{{s}}}

1. pAF125

   Plasmid

   #222373

   Purpose

   Recombinant human IgG antibody vector without variable regions. Contains mTagBFP marker.

   Depositor

   Andrew S. Flies

   Article

   Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

   Insert

   IGHG (partial)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAF146

   Plasmid

   #222377

   Purpose

   Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

   Depositor

   Andrew S. Flies

   Article

   Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

   Insert

   PDL1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF201

   Plasmid

   #222376

   Purpose

   Recombinant Tasmanian devil IgG antibody (1F8) that binds to Tasmanian devil PDL1

   Depositor

   Andrew S. Flies

   Article

   Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

   Insert

   IGHG
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF200

   Plasmid

   #222375

   Purpose

   Recombinant Tasmanian devil IgG antibody (3D5) that binds to 6xHis at the C-terminus of proteins

   Depositor

   Andrew S. Flies

   Article

   Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

   Insert

   IGHG
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

   Plasmid

   #217514

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

   Plasmid

   #217509

   Purpose

   KD Recombinase dependent conditional 1X ALFA cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
   

   Use

   Tags

   ALFA tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mAMCase_CatD_6xHis_pcDNA3.1

   Plasmid

   #200229

   Purpose

   Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

   Depositor

   James S Fraser

   Article

   Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

   Insert

   Acidic Mammalian Chitinase (Chia1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S354F

   Promoter

   CMV

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Crimson/P2A-Control (pSLIK3)

   Plasmid

   #113858

   Purpose

   An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   E2-Crimson
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSP1D12W/2Y

   Plasmid

   #124275

   Purpose

   Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence

   Depositor

   Alexey S. Ladokhin

   Insert

   Membrane scaffold protein
   

   Use

   Tags

   7xHis-tag

   Expression

   Bacterial

   Mutation

   Two tryptophans changed to two tyrosines

   Promoter

   T7

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-roGFP1-iR

    Plasmid

    #83314

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iR
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

    Promoter

    T5

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pRSETB-ro3GFP

    Plasmid

    #82367

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro3GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/N149C/S202C

    Promoter

    T7

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-Jagged [D5] (Recombinant Antibody)

    Antibody

    #201864-rAb

    Purpose

    Anti-Jagged (Human) recombinant scFv-Fc-fusion

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Recommended Applications

    Immunocytochemistry

    Reactivity

    Human

    Source Species

    Human

    Isotype

    IgG1

    Trial Size

    Available to purchase

    Available since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

13. Epac-S-H187

    Plasmid

    #170348

    Purpose

    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

    Insert

    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Epac-S-H170

    Plasmid

    #170352

    Purpose

    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

    Insert

    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Epac-S-H81

    Plasmid

    #170339

    Purpose

    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0079 RESCUE-S

    Plasmid

    #130662

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX_TRC311 ETV4-S

    Plasmid

    #74983

    Purpose

    Constitutive ETV4 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 )
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAB1923 REPAIR.t1-S

    Plasmid

    #176321

    Purpose

    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q/E620G/Q696L, only the deaminase domain (aa …

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4 huZAP(S)

    Plasmid

    #45906

    Depositor

    Harmit Malik

    Article

    Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

    Insert

    ZAP(S) isoform (ZC3HAV1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    M202T, L388F

    Promoter

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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