Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 22,626 results for: BASE;

Showing: 101 - 120 of 22626 results

  1. Desmoplakin II acceptor only control (F40-based tension sensor)

    Plasmid
    #118719
    Purpose
    The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)(Y67G)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118719-plasmid-map-sequence-id-230445

    Enlarge

  2. Desmoplakin II acceptor only control (F40-based no force control)

    Plasmid
    #118720
    Purpose
    The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y67G mutation in YPet(sh…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118720-plasmid-map-sequence-id-230457

    Enlarge

  11. GFP-itis Bacterial Base Editor Activity

    Plasmid Kit
    #1000000219
    Purpose
    This collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.
    Depositor
    Alexis Komor
    Available since
    May 1, 2023
    Availability
    Academic Institutions and Nonprofits only

  12. pRDB_270

    Plasmid
    #231111
    Purpose
    C>T base editor
    Depositor
    John Doench, David Root
    Insert
    TadA-CDd-nCas9-SpG-UGI-HA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    231111-plasmid-map-sequence-id-458638

    Enlarge

  13. pRDA_867

    Plasmid
    #231109
    Purpose
    A>G base editor
    Depositor
    John Doench, David Root
    Insert
    ABE8e-nCas9-SpG-HA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231109-plasmid-map-sequence-id-458425

    Enlarge

  14. pRDB_092

    Plasmid
    #231110
    Purpose
    C>T base editor
    Depositor
    John Doench, David Root
    Insert
    TadA-CDd-nCas9-SpG-UGI
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231110-plasmid-map-sequence-id-459684

    Enlarge

  16. AYBEv0.1

    Plasmid
    #193966
    Purpose
    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, EF1a, CBh
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    193966-plasmid-map-sequence-id-384658

    Enlarge

Showing: 101 - 120 of 22626 results