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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 260 results for: G2

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Showing: 101 - 120 of 260 results

  1. ATP6 alphaDdCBE G2

    Plasmid
    #221959
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Publication
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A, See depositor comments
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221959-plasmid-map-sequence-id-438714

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  3. pcDNA5 FRT TO GFP hBub1 WT

    Plasmid
    #59812
    Purpose
    Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.
    Depositor
    Stephen Taylor
    Publication
    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bub1 (BUB1 Human)
    Tags
    EmGFP
    Expression
    Mammalian
    Promoter
    hybrid human cytomegalovirus (CMV)/TetO2 promoter
    Available since
    Feb. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    59812-plasmid-map-sequence-id-99116

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  6. pcDNA5 FRT TO GFP hBub1 K821R

    Plasmid
    #59813
    Purpose
    Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.
    Depositor
    Stephen Taylor
    Publication
    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bub1 (BUB1 Human)
    Tags
    EmGFP
    Expression
    Mammalian
    Mutation
    K821R
    Promoter
    hybrid human cytomegalovirus (CMV)/TetO2 promoter
    Available since
    Feb. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    59813-plasmid-map-sequence-id-99117

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  7. pGMC00022 (aka pRC0433)

    Plasmid
    #195321
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with hygromycin selection
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195321-plasmid-map-sequence-id-385749

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  9. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid
    #217662
    Purpose
    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217662-plasmid-map-sequence-id-440854

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  10. pcDNA5 FRT TO GFP hBub1 WT

    Plasmid
    #59812
    Purpose
    Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.
    Depositor
    Stephen Taylor
    Publication
    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bub1 (BUB1 Human)
    Tags
    EmGFP
    Expression
    Mammalian
    Promoter
    hybrid human cytomegalovirus (CMV)/TetO2 promoter
    Available since
    Feb. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    59812-plasmid-map-sequence-id-99116

    Enlarge

  13. pcDNA5 FRT TO GFP hBub1 K821R

    Plasmid
    #59813
    Purpose
    Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.
    Depositor
    Stephen Taylor
    Publication
    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bub1 (BUB1 Human)
    Tags
    EmGFP
    Expression
    Mammalian
    Mutation
    K821R
    Promoter
    hybrid human cytomegalovirus (CMV)/TetO2 promoter
    Available since
    Feb. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    59813-plasmid-map-sequence-id-99117

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  14. AAVS1_Cas9-hGem_gRNA2

    Plasmid
    #217659
    Purpose
    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA2 targeting the human AAVS1 safe harbour locus
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217659-plasmid-map-sequence-id-438226

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  16. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Publication
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107999-plasmid-map-sequence-id-217151

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  19. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid
    #98975
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 4 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98975-plasmid-map-sequence-id-199218

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  20. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Publication
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107999-plasmid-map-sequence-id-217151

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Showing: 101 - 120 of 260 results