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Plasmid#60834PurposeMouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRESDepositorInsertNrasG12D (Nras Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
R777-E146 Hs.NRAS-nostop
Plasmid#70430PurposeGateway ORF clone of human NRAS [NM_002524.4] without stop codon (for C-terminal fusions)DepositorInsertNRAS (NRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K-IRES-Puro
Plasmid#120570PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61KPromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRAS-IRES-Puro
Plasmid#120569PurposeExpresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FLAG-NRASQ61K-IRES-Puro
Plasmid#120575PurposeExpresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFLAGExpressionMammalianMutationQ61KPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FLAG-NRAS-IRES-Puro
Plasmid#120574PurposeExpresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K/T35A-IRES-Puro
Plasmid#120571PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, T35APromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K/Y40A-IRES-Puro
Plasmid#120573PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, Y40APromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K/E37A-IRES-Puro
Plasmid#120572PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, E37APromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKC12 - DelQ
Plasmid#110493PurposeMonitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.DepositorInsertNRAS 5'- DelQ (bp 30-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationDeletion of the first 29 base pairs of the NRAS 5…PromoterT7Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKC11 - UTRQ
Plasmid#110490PurposeMonitor the expression of Luciferase driven by the NRAS 5'-UTR.DepositorInsertNRAS 5'-UTR (bp 1-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationPromoterT7Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKC12 - DelQ
Plasmid#110493PurposeMonitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.DepositorInsertNRAS 5'- DelQ (bp 30-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationDeletion of the first 29 base pairs of the NRAS 5…PromoterT7Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKC11 - UTRQ
Plasmid#110490PurposeMonitor the expression of Luciferase driven by the NRAS 5'-UTR.DepositorInsertNRAS 5'-UTR (bp 1-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationPromoterT7Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe N-Ras 61K
Plasmid#12543DepositorInsertNRas 61K (NRAS Human)
UseRetroviralTagsExpressionMammalianMutation61K constitutively activated.PromoterAvailable sinceSept. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2FP
Plasmid#203757PurposeEncodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorInsertNRas c-term (NRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe N-Ras 61K
Plasmid#12543DepositorInsertNRas 61K (NRAS Human)
UseRetroviralTagsExpressionMammalianMutation61K constitutively activated.PromoterAvailable sinceSept. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
Cag.Luc.puro
Plasmid#74409PurposeConstitutive Luciferase expression in mammalian cells along with puromycin selectionDepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro
Plasmid#120564PurposeExpresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationQ61KPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro
Plasmid#120563PurposeExpresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro
Plasmid#120565PurposeExpresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationQ61K, T35APromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only