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Plasmid#45309DepositorInsertMCOPS3 (SOX2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4B (205-224) (pGEX2T(TEV))
Plasmid#80634PurposeResidues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201DepositorInsertCHMP4B (CHMP4B Human)
UseTagsGSTExpressionBacterialMutationResidues 205-224PromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) E209A (pGEX2T(TEV))
Plasmid#80640PurposeResidues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationChanged Glutamate 209 to alaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCA528 CHMP4A 153-222
Plasmid#108256PurposeExpresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6DepositorInsertCHMP4A residues 153-222 (CHMP4A Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) L217A (pGEX2T(TEV))
Plasmid#80638PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Leucine 217 to AlaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) W220A (pGEX2T(TEV))
Plasmid#80637PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Trp 220 to AlaPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) L214A (pGEX2T(TEV))
Plasmid#80639PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Leucine 214 to AlaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-HT-LC3-SV40-Hygro
Plasmid#182045PurposeLentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closureDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsHaloTag-TEVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-976aa)
Plasmid#139689PurposeExpresses N-teminal Flag tagged aa 1-976 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-360aa)
Plasmid#139668PurposeExpresses N-teminal Flag tagged aa 1-360 of LARS1 proteinDepositorInsertLeucyl-tRNA Synthetase (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCHMP4B-NS3-Blue
Plasmid#223534PurposeMammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.DepositorInsertCHMP4B (CHMP4B Human)
UseTagsNS3 Hepatitis C protease and mTurquoiseExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP4B-mut-NS3-Green
Plasmid#223531PurposeMammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP4B (CHMP4B Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP4B-NS3-Green
Plasmid#223528PurposeMammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP4B (CHMP4B Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4B(S184C)
Plasmid#180026PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).DepositorInsertGB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS184CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-720aa)
Plasmid#139687PurposeExpresses N-teminal Flag tagged aa 1- 720 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(721-1176aa)
Plasmid#139690PurposeExpresses N-teminal Flag tagged aa 721-1176 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS-DELTA-UNE-L
Plasmid#139691PurposeExpresses N-teminal Flag tagged aa 1- 1064 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4B 156-224
Plasmid#104596PurposeExpresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7DepositorInsertCHMP4B residues 156-224 (CHMP4B Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only