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pJW1514

Plasmid

#62384

Purpose

pCR2.1 linker-tev-linker-3X-FLAG::KanMX

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

tev 3x FLAG

Use

Yeast c-terminal tagging vector

Tags

linker-tev-linker-3xflag

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAct1IHPT-4

Plasmid

#37844

Depositor

Peggy Lemaux

Article

Myeong-Je et al Volume 138, Issue 2, 23 November 1998, P

Insert

Actin promoter and intron

Use

Plant transformation

Tags

HPT (Hygromycin phosphotransferase) and nos 3…

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-ALDH1A3

Plasmid

#140589

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA ALDH1A3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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MSP971

Plasmid

#98805

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-ENLYFQS-TEV E107D, D127A, S135F, R203Q, K215E

Expression

Bacterial

Mutation

E107D, D127A, S135F, R203Q, K215E

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pmRuby2-IRES-Clover

Plasmid

#171059

Purpose

Expresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET.

Depositor

Ke Xu

Article

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

IRES

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSpec

Plasmid

#60593

Purpose

used with pAND_YFP to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Spec

Expression

Bacterial

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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CUP Snf5 YFP p316

Plasmid

#15597

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

SNF5 (SNF5 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCis_7+8+10

Plasmid

#60588

Purpose

a 3-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8+10

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCis_7+8

Plasmid

#60587

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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PPARdelta KO vector

Plasmid

#16619

Depositor

Bert Vogelstein

Article

Park et al Proc Natl Acad Sci U S A. 2001 Feb 27. 9

Insert

PPAR delta (PPARD Human)

Expression

Bacterial

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-SYNPO

Plasmid

#140587

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA SYNPO

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1671

Plasmid

#112045

Purpose

CEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ORM1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCis_2+5

Plasmid

#60586

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+5

Expression

Bacterial

Promoter

pBAD

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1675

Plasmid

#112049

Purpose

CEN/ARS plasmid for GAL-inducible TSC13-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

TSC13

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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GST-PRMT7-ΔN

Plasmid

#34694

Depositor

Steven Clarke

Article

Miranda et al J Biol Chem. 2004 May 28;279(22):22902-7

Insert

PRMT7 (PRMT7 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains aa 379–692

Promoter

tac

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only
pOX-twk24

Plasmid

#16120

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel (twk-24 Nematode)

Use

Xenopus oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pJW1516

Plasmid

#62386

Purpose

pFA6a Osm1(M1A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 1 to Alanine

Promoter

Adh1p

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBSc-twk31

Plasmid

#16126

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
sVRC01 1138 HL pITR

Plasmid

#251813

Purpose

pITR plasmid encoding simianized VRC01 bnAb HC and LC with endogenous rhesus macaque elements for production as AAV6 for engineering into rhesus macaque IgH locus

Depositor

James Voss

Insert

simianized VRC01

Use

AAV

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pDASH-DIS-α2 (MoClo)

Plasmid

#242524

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, compatible with MoClo, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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