We narrowed to 1,496 results for: MBP;

1. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Use

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSL103-SpCas9-FLAG-3XNLS

   Plasmid

   #182032

   Purpose

   Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

   Depositor

   Steven Lin

   Article

   Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

   Insert

   Streptococcus pyogenes cas9 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   12X His tag, 3XNLS, FLAG, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-APOBEC1-YTH

   Plasmid

   #200158

   Purpose

   For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTH
   

   Use

   Tags

   6xHis, HA, and Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   LacZ

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ubiquitin K48C

   Plasmid

   #86588

   Purpose

   Bacterial expression of Ubiquitin K48C for cross linking studies

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; K48C

   Promoter

   Tac

   Available since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pJCC_083 Cas12i1

   Plasmid

   #171670

   Purpose

   For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Ubiquitin K63C

   Plasmid

   #86589

   Purpose

   Bacterial expression of Ubiquitin K63C for cross linking studies

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; K63C

   Promoter

   Tac

   Available since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pMJ823

   Plasmid

   #39313

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (lin2744 Listeria innocua)
   

   Use

   CRISPR

   Tags

   MBP and thioredoxin-His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pHIT179

   Plasmid

   #204510

   Purpose

   Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

   Depositor

   Yuji Kohara

   Article

   Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

   Insert

   csr-1a (C. elegans) (csr-1 Nematode)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMTTH+mEGFP-LANA

   Plasmid

   #157801

   Purpose

   Expression of mEGFP and LANA peptide fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   mEGFP-LANA
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dhh1-EGFP

    Plasmid

    #201418

    Purpose

    Bacterial protein expression of Dhh1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMAL-c2x-MT3

    Plasmid

    #32115

    Depositor

    Chris Mercogliano, Cristina Risco Ortiz, David DeRosier

    Article

    Diestra et al J Struct Biol. 2009 Mar;165(3):157-68. E

    Insert

    MT (3x) (Mt1 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    P-lac

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Ubiquitin G76C

    Plasmid

    #86583

    Purpose

    Bacterial expression of Ubiquitin G76C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; G76C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Rag2 T490A

    Plasmid

    #115650

    Purpose

    expression of full length Rag2 with T490A mutation

    Depositor

    Martin Gellert

    Article

    Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

    Insert

    Rag2 (Rag2 Mouse)
    

    Use

    Tags

    8XHis-MBP-PreScission

    Expression

    Mammalian

    Mutation

    T490A

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM41-EcPPXc

    Plasmid

    #38329

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pRK1036

    Plasmid

    #17728

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    NusG (nusG Aquifex aeolicus)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHPK1212

    Plasmid

    #8834

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    tobacco vein mottling virus protease, catalytic domain
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMTTH+bromo5

    Plasmid

    #157803

    Purpose

    Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    bromo5
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ubiquitin K6C

    Plasmid

    #86587

    Purpose

    Bacterial expression of Ubiquitin K6C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K6C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pRC04

    Plasmid

    #114683

    Purpose

    FRB-TEV-N

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Insert

    FRB-TEV-N
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPZP FLIPmal-25u

    Plasmid

    #14476

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPmal
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    W230A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only