We narrowed to 1,687 results for: plasmid for e coli

1. pET11a_3xFlag-nsp14 (SARS-CoV-2)

   Plasmid

   #169159

   Purpose

   To express SARS-CoV-2 nsp14 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11a_3xFlag-nsp10 (SARS-CoV-2)

   Plasmid

   #169157

   Purpose

   To express SARS-CoV-2 nsp10 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE-RNaseHI-D10R-E48R-NLS-mCherry

   Plasmid

   #60367

   Purpose

   Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   RNase HI-D10R-E48R
   

   Use

   Tags

   NLS from SV40 T antigen and mCherry

   Expression

   Mammalian

   Mutation

   D10R+E48R; catalyticaly inactive

   Promoter

   CMV-tet

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-ABE-NT-sgRNA

   Plasmid

   #112734

   Purpose

   AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

   Depositor

   Jin-Soo Kim

   Article

   Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

   Insert

   ABE-NT-sgRNA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pλPR cI-Elysis

   Plasmid

   #176891

   Purpose

   Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

   Depositor

   James Inglese

   Article

   Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

   Insert

   Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST_116_LVL2 cam

   Plasmid

   #179332

   Purpose

   NT-CRISPR plasmid for a single gRNA.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST_119_LVL2 cam

   Plasmid

   #179333

   Purpose

   NT-CRISPR plasmid for integration of multiple gRNAs.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL.028

   Plasmid

   #184965

   Purpose

   Test effect of extending -Eco1 a1/a2 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(extended), a1/a2 length: 27
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   human codon optimized RT, a1/a2 length extended t…

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSO221 Phis-72 BirA GFP

   Plasmid

   #45100

   Depositor

   Steven Henikoff

   Article

   Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

   Inserts

   BirA::GFP
   unc-119 (unc-119 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   his-72 and unc-119

   Available since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-tTA

    Plasmid

    #193338

    Purpose

    AAV-mediated expression of tTA under the CAG promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSKA397

    Plasmid

    #80380

    Purpose

    mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    cat
    metE
    

    Use

    Synthetic Biology; Mini-tn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat promoter and cpcG2Δ59

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR)

    Plasmid

    #113762

    Purpose

    AAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

    Depositor

    Luke Lavis

    Article

    Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

    Insert

    tdNfsB(F124W)-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F124W

    Promoter

    CAG

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. aav-SYN-FLEX-tdNfsB(F124W)-mCherry (eNTR)

    Plasmid

    #113763

    Purpose

    AAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

    Depositor

    Luke Lavis

    Article

    Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

    Insert

    tdNfsB(F124W)-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F124W

    Promoter

    Synapsin

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC234r-ccdB/TPK2

    Plasmid

    #176179

    Purpose

    yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    dropout cassette containing toxic genes ccdB and TPK2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE76

    Plasmid

    #80578

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE77

    Plasmid

    #80579

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGE78

    Plasmid

    #80580

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGE79

    Plasmid

    #80581

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFP121

    Plasmid

    #21670

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pWR88

    Plasmid

    #203422

    Purpose

    empty BLINCAR plasmid for payload delivery, no payload, no genomic target

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    CAR copy 1
    P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
    CAR copy 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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