We narrowed to 1,719 results for: plasmid for e coli

1. MP-U2

   Plasmid

   #69700

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 RBS+ dam seqA emrR ugi CDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available Since

   Sept. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-PsppA-mCherry

   Plasmid

   #225428

   Purpose

   Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

   Depositor

   Geir Mathiesen

   Article

   Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

   Insert

   mCherry, Cas9, SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PsppA upstream of mCherry and Cas9, P3 upstream o…

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL NarX Myc

   Plasmid

   #225651

   Purpose

   Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NarX (narX E. coli)
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. aav-CAG-tdNfsB(F124W)-mCherry (eNTR)

   Plasmid

   #113761

   Purpose

   AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

   Depositor

   Luke Lavis

   Article

   Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

   Insert

   tdNfsB(F124W)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F124W

   Promoter

   CAG

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. aav-SYN-tdNfsB(F124W)-mCherry (eNTR)

   Plasmid

   #113760

   Purpose

   AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

   Depositor

   Luke Lavis

   Article

   Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

   Insert

   tdNfsB(F124W)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F124W

   Promoter

   Synapsin

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-BirA-CPDSalI

   Plasmid

   #38258

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   birA (birA E. coli)
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pGEX4T1-BirA

   Promoter

   T7

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pFP122

   Plasmid

   #21669

   Depositor

   James Haber

   Article

   Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

   Insert

   Lacz (lacZ E. coli)
   

   Use

   Centromeric plasmid

   Tags

   Expression

   Mutation

   The second lacZ sequence has been modified, the E…

   Promoter

   Available Since

   Sept. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pSCL.103

    Plasmid

    #184988

    Purpose

    Express Cas9-P2A-Eco1RT

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCL.027

    Plasmid

    #184964

    Purpose

    Express -Eco1 RT and ncRNA in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(wt), a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.102

    Plasmid

    #184987

    Purpose

    Express -Eco1 RT-P2A-Cas9

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-P2A-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCL.006

    Plasmid

    #184970

    Purpose

    Express -Eco1 RT in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Integrating inducible cassette: Eco1 RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid

    #218514

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(1)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid

    #218778

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(3)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIA1385

    Plasmid

    #166862

    Purpose

    Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGG-TurboID-GFP-PTS1

    Plasmid

    #209406

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID-PTS1
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. WT FL USP7

    Plasmid

    #227214

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEVA3b67Rb

    Plasmid

    #58311

    Purpose

    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX4T-3-6AOptParkin

    Plasmid

    #165107

    Purpose

    bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene

    Depositor

    Arthur Haas

    Insert

    Parkin isoform 1 (PRKN Human)
    

    Use

    Tags

    GST-6-alanine

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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