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pcDNA3-HA-BHLHE40(S243A/E248A)

Plasmid

#110155

Purpose

Expression of human BHLHE40 (S243A/E248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Mutation

S243A/E248A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-ZYG11A

Plasmid

#110551

Purpose

expresses a FLAG-tagged human ZYG11A protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11A (ZYG11A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCOND (Apr)

Plasmid

#110094

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Apr)

Plasmid

#110078

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-pduCDE

Plasmid

#110138

Purpose

Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

Insert

pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)

Expression

Bacterial

Promoter

T7

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-eEF2K(S441A/S445A)

Plasmid

#110161

Purpose

Expression of human eEF2K (S441A/S445A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

Insert

eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)

Tags

HA

Expression

Mammalian

Mutation

S441A/S445A

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCZ829

Plasmid

#110137

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Article

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito-GCaMP5

Tags

mito::GCaMP5

Expression

Worm

Promoter

Pcol-19

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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hCCR5-LSSmOrange

Plasmid

#110194

Purpose

Encodes for human CCR5 coding sequence tagged with the LSS-mOrange FP

Depositor

Sergi Padilla Parra

Article

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Insert

hCCR5 (CCR5 Human)

Tags

LSSmOrange

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314-hUI

Plasmid

#11006

Depositor

Francisco Malagon

Insert

hUI recombination system

Expression

Bacterial and Yeast

Mutation

Inverted repeat recombination system. Recombinati…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2 227X

Plasmid

#11050

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 227X (CDC73 Human)

Tags

flag

Expression

Mammalian

Mutation

truncation mutant with a stop codon introduced at…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3 HRPT2 L64P

Plasmid

#11052

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 L64P (CDC73 Human)

Tags

Flag

Expression

Mammalian

Mutation

L64P

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
p116 Mfz10a

Plasmid

#11070

Depositor

Ramesh Shivdasani

Article

Malik et al Biochem J. 2000 Aug 1;349 Pt 3(Pt 3):829

Insert

Mfz10a (Fzd10 Mouse)

Expression

Bacterial

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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p029 End-1::EnR

Plasmid

#11029

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Dominant inhibitory GATA-binding protein End1::En…

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP3-His

Plasmid

#67871

Purpose

bacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP3 (PRORP3 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.puro_shHuR CDS

Plasmid

#110411

Purpose

TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

Depositor

Sandra Martha Gomes Dias

Insert

ELAVL1 (HuR)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

Plasmid

#110300

Purpose

Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK107

Plasmid

#110575

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP25 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK121

Plasmid

#110581

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

PA3 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK403

Plasmid

#110599

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBMN-mEGFP-C1

Plasmid

#110468

Purpose

retrovirus construct for stable expressing mEGFP tagged protein

Depositor

Richard Youle

Article

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

mEGFP

Use

Retroviral; Retrovirus

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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