Addgene

We narrowed to 10,505 results for: CHL

Showing: 1201 - 1220 of 10505 results

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pAAV-CAG-soCoChR-GFP

Plasmid

#107710

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-soCoChR-mCardinal

Plasmid

#107713

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-mCardinal

Use

AAV

Tags

KA2 and mCardinal

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_LTN1

Plasmid

#127125

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA LTN1 (LTN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_CELF1

Plasmid

#106099

Purpose

Inducible gene expression of FlagHA - CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA Ago2

Plasmid

#10821

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

R668H. Additionally, N terminus different from wi…

Promoter

Available since

Oct. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-GFP

Plasmid

#107709

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis PL-T500

Plasmid

#92314

Purpose

Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

B1 domain of protein L (PL)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ELAVL2

Plasmid

#106107

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL2 (ELAVL2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p70b-UTR1-deGFP-6xHis-T500

Plasmid

#92222

Purpose

Expression of deGFP using the p70b promoter (weak version of the p70a promoter)

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

p70b (p70a promoter with one mutation)

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)_G

Plasmid

#146407

Purpose

Bacterial Expression of HsDCP1a_539-582

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582 (DCP1A Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation T1716G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_G

Plasmid

#146409

Purpose

Bacterial Expression of HsDCP1a_539-582-mut2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582-mut2 (DCP1A Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation T1716G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_G

Plasmid

#146411

Purpose

Bacterial Expression of HsDCP1a_539-582

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582 (DCP1A Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation T1716G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK_E

Plasmid

#146211

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-E236Q_E

Plasmid

#146212

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-E236Q (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-K135A_E

Plasmid

#146213

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-K135A (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E

Plasmid

#146215

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-E236Q (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E

Plasmid

#146216

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-K135A (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmTral_1-84_C

Plasmid

#146082

Purpose

Bacterial Expression of DmTral_1-84

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_1-84 (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation A198C compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E

Plasmid

#146204

Purpose

Insect Expression of DmMe31B-Q281AH284A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-Q281AH284A (me31B Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E

Plasmid

#146205

Purpose

Insect Expression of DmMe31B-T288AK292A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-T288AK292A (me31B Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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