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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,492 results for: PUC19

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Showing: 1201 - 1220 of 2492 results

  5. pBV514

    Plasmid
    #204602
    Purpose
    Expresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoter
    Depositor
    Scott Moye-Rowley
    Insert
    Os Tir1 (LOC4335696 Oryza sativa)
    Tags
    Myc epitope tags
    Expression
    Bacterial
    Mutation
    Changed phenylalanine 74 into an alanine (F74A).
    Promoter
    ScADH1
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204602-plasmid-map-sequence-id-417406

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  6. pIAeBlue

    Plasmid
    #185891
    Purpose
    In vivo gene amplification (HapAmp) of AeBlue expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185891-plasmid-map-sequence-id-370863

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  9. pISL1-donor

    Plasmid
    #113820
    Purpose
    CRISPR donor plasmid to tag human transcription factor ISL1 with GFP
    Depositor
    Kevin White
    Insert
    ISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113820-plasmid-map-sequence-id-230188

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  10. pERF-donor

    Plasmid
    #112299
    Purpose
    CRISPR donor plasmid to tag human transcription factor ERF with GFP
    Depositor
    Kevin White
    Insert
    ERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
    Use
    CRISPR
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112299-plasmid-map-sequence-id-228410

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  17. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  18. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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  20. pTSHZ2-donor

    Plasmid
    #113810
    Purpose
    CRISPR donor plasmid to tag human transcription factor TSHZ2 with GFP
    Depositor
    Kevin White
    Insert
    TSHZ2 homology arms flanking EGFP-IRES-Neo cassette (TSHZ2 Human)
    Use
    CRISPR
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113810-plasmid-map-sequence-id-229895

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Showing: 1201 - 1220 of 2492 results