We narrowed to 26,884 results for: STI

1. ATP6 DdCBE A1

   Plasmid

   #221946

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP6 DdCBE A2

   Plasmid

   #221947

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PYPQ121-DdCBE

   Plasmid

   #218174

   Purpose

   Express plastid DdCBE, composed of TALE, split DddA originated from Burkholderia cenocepacia and UGIs

   Depositor

   Yiping Qi

   Article

   Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

   Insert

   TALE-Intron-N-DddAtox half (G1397N)-UGI-T2A-TALE-C-DddAtox half (G1397C)-UGI
   

   Use

   Plastid base editing

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWR95(URA3)

   Plasmid

   #203917

   Purpose

   BLINCAR plasmid for gene deletion, URA3 target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   URA3 upstream homology
   URA3 downstream homology
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-miniTurbo-V5-PRPF4B_IDG-K

   Plasmid

   #214463

   Purpose

   Gateway destination clone of PRPF4B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-PRPF4B (PRPF4B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-STK19_IDG-K

   Plasmid

   #214465

   Purpose

   Gateway destination clone of STK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-STK19 (STK19 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWR95

   Plasmid

   #203916

   Purpose

   BLINCAR plasmid for gene deletion, no genomic target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   modified MCS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3VEA3

   Plasmid

   #234796

   Purpose

   Mammalian expression of bovine arrestin-3

   Depositor

   Vsevolod Gurevich

   Article

   Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

   Insert

   Arrestin-3 (ARR3 Bovine)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. tFucci(CA)5

    Plasmid

    #153521

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(CA)5.
    

    Use

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSiMPlk_N + pSiMPlk_C

    Plasmid

    #134310

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. tFucci(SA)5

    Plasmid

    #153520

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(SA)5.
    

    Use

    Tags

    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TL1 DdCBE A1 (DddA6-C)

    Plasmid

    #221992

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TL1 DdCBE T2 (DddA6-N)

    Plasmid

    #221993

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: Q1310R, S1330I, T1380I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pABM-D4-miR30-CypA

    Plasmid

    #132934

    Purpose

    Lentivector for CypA knockdown and blasticidin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    CypA shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV 4xSTAR-mNeonGreen-NLS-blast

    Plasmid

    #136258

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV 4xSTAR-sTomato-NLS-blast

    Plasmid

    #136259

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

    Plasmid

    #113620

    Purpose

    sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7a sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

20. qi5

    Plasmid

    #24501

    Depositor

    Bruce Conklin

    Article

    Conklin et al Nature. 1993 May 20. 363(6426):274-6.

    Insert

    Gq alpha (Gnaq Mouse)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    Gq alpha with the C-terminal amino acids changed …

    Promoter

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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