We narrowed to 7,293 results for: ef1a

1. pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

   Plasmid

   #134859

   Purpose

   Expresses EGFP-tagged ERGIC marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   ERGIC53 (Lman1 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRH-1363 LV EF1a-CD19 IRES-EGFP

   Plasmid

   #201919

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

   Plasmid

   #234950

   Purpose

   Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-Flex-MCS-WPRE-SV40 - GCH14

   Plasmid

   #230821

   Purpose

   AAV cloning vector for CRE-dependent expression under the EF1a promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #125691

   Purpose

   Global labeling of excitatory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

   Plasmid

   #203842

   Purpose

   Flp-dependent Cre and EGFP expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   EGFP-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

   Plasmid

   #87306

   Purpose

   An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre-dependent mouse codon-optimized Flp recombinase.
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

   Plasmid

   #203847

   Purpose

   Flp-dependent expression of Chronos fused to EGFP

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   Chronos
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a-FRT-MCS-WPRE-SV40 - GCH15

   Plasmid

   #230822

   Purpose

   AAV cloning vector for Flpo-dependent expression under the EF1a promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV; Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-tetOn-IRES-G418 (EtO)

    Plasmid

    #84776

    Purpose

    Second generation lenti tetracycline operator (on)

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Insert

    TET OPERATOR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTIGHT

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

    Plasmid

    #130998

    Purpose

    Double floxed ChRmine-mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

    Plasmid

    #37082

    Purpose

    Cre-dependent expression of hChR2(H134R) for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

    Insert

    hChr2(H134R)-mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a DIO-rsChRmine-oScarlet-WPRE

    Plasmid

    #183528

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Insert

    rsChRmine-oScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I146M/G174S

    Promoter

    Ef1a

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-UCOE-EF1a-Zim3-dCas9-BFP

    Plasmid

    #188775

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

    Plasmid

    #201681

    Purpose

    Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

    Depositor

    Emma Rawlins

    Article

    Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

    Inserts

    SFTPC promoter region (2kb upstream)
    TagRFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167942

    Purpose

    Inducible NKX2-1 overexpression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    TTF1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

    Plasmid

    #173029

    Purpose

    For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    pspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, BFP, HIV NES, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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