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Plasmid#52522Purposeexpresses a myc-tagged version of hCas9 in DrosophilaDepositorInsertS. pyogenes cas9 with humanized codon bias
UseTagsmycExpressionInsectMutationPromotertubulinAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2iApowriter2(#336)
Plasmid#184104Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesisTagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2iU6Apowriter2N(#355)
Plasmid#184106Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesisDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesis + in vitro trans…Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-Ubi+SP6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Apowriter2(#340)
Plasmid#184105Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
UseTagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas3
Plasmid#178881PurposeExpression of human codon-optimized NlaCas3 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas3c-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas5
Plasmid#178878PurposeExpression of human codon-optimized Nlacas5 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas5c-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas7
Plasmid#178879PurposeExpression of human codon-optimized Nlacas7 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas7c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP no-stop
Plasmid#123209PurposeGateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C no-stop
Plasmid#123208PurposeGateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTV001
Plasmid#130265PurposeEatA expression plasmid containing 8His encoding region in permissive site of eatA geneDepositorInserteatA::His8
UseTagspolyhistidine (internal)ExpressionBacterialMutation8His polyhistidine encoding region and XhoI site …PromoteraraBADAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMS
Plasmid#51543PurposeInducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2DepositorUseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVimentin-LSSmOrange
Plasmid#37134DepositorInsertLSSmOrange
UseTagshuman cytochrome C oxidase subunit VIII and human…ExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMSOX17FNV
Plasmid#206382PurposeExpresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-62
Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+CMV-Ins-GLuc
Plasmid#89929PurposeExpresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…PromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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9336-E24
Plasmid#234307PurposeGateway ORF Entry clone of human SMAD3 truncated with stop codon (for native or N-terminal fusions); C-term truncation to delete phosphorylation sitesDepositorInsertSMAD3 truncated (SMAD3 Human)
UseGateway entry cloneTagsExpressionMutationdel(422-425); C-term truncation to delete phospho…PromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only