We narrowed to 1,735 results for: nif

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pCRISPR

Plasmid

#186276

Purpose

Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira shortened CRISPR array

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSin4 UAP1 F383G

Plasmid

#169891

Purpose

Used for generation of stable mammalian cell lines expressing UAP1 with with F383G mutation

Depositor

Jennifer Kohler

Article

Capota et al Curr Protoc. 2021 Jul;1(7):e201. doi: 10

Insert

UDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Changed Phenylalanine (F) at position 383 to glyc…

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1141

Plasmid

#160867

Purpose

PJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Coniferyl alcohol acyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S + STS:P4S

Plasmid

#139789

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

biosynthetic enzymes 4CL and STS in fusion with coiled-coil segments

Expression

Bacterial

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAW218

Plasmid

#113240

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-MTS2 (12)

Plasmid

#101698

Purpose

MTS2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA7

Plasmid

#83871

Purpose

Gateway donor vector for IAA7

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA7 (IAA7 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA8

Plasmid

#83873

Purpose

Gateway donor vector for IAA8

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA8 (IAA8 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA31

Plasmid

#83864

Purpose

Gateway donor vector for IAA31

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA31 (IAA31 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA16

Plasmid

#83854

Purpose

Gateway donor vector for IAA16

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA16 (IAA16 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

Plasmid

#39306

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E211A, K212A, E213A, R214A, D215A and Y217A

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pJRH-1328 LV EF1a-CD20 IRES-EGFP

Plasmid

#201918

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD20

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1187 VSVGmut

Plasmid

#201913

Purpose

Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

VSV-G (K47Q, R354A)

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Has Service

Cloning Grade DNA

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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c-Fos

Plasmid

#187905

Purpose

Expresses c-Fos in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

cFos (FOS Human)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDAC565

Plasmid

#195242

Purpose

Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Article

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Ptgs2

Plasmid

#209519

Purpose

Expresses luciferase under control of Ptgs2 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Ptgs2 (Ptgs2 Mouse)

Use

Luciferase

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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c-Jun

Plasmid

#187902

Purpose

Expresses c-Jun in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

c-Jun (JUN Human)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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