We narrowed to 6,822 results for: pcdna3.1

Showing: 1201 - 1220 of 6822 results

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ZoAl-ENT N-1F (R1103A)

Plasmid

#216548

Purpose

For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Tags

Flag

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Mutation

Deleted the partial 3'ribozyme sequence in T…

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Clover-Prx2-mRuby2

Plasmid

#117906

Purpose

fluorescent reporter of cytosolic hydrogen peroxide

Depositor

Hadley Sikes

Article

Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

Insert

Clover-Prx2-mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

Plasmid

#52502

Purpose

expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

deletion of Fbox (deleted aa 71-116)

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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Venus-G protein Beta2 in pcDNA3.1

Plasmid

#42182

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Beta 2 (GNB2 Human)

Tags

Venus(156-239)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-qmTQ2-cAMP

Plasmid

#225250

Purpose

mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (R232)

Plasmid

#214152

Purpose

Transient expression of STING R232

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (STING1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR3c-V5/HIS

Plasmid

#201108

Purpose

expression of human FGFR3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/ZMYM2-FGFR1

Plasmid

#167486

Purpose

Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

Depositor

Nicholas Cross

Article

Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

Insert

ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-AKAP95-FRB

Plasmid

#138254

Purpose

Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

Depositor

Jin Zhang

Article

Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

Insert

AKAP95-FRB (AKAP8 Human)

Tags

FRB rapamycin binding domain and HA epitope

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_V5-ANKRD52

Plasmid

#92012

Purpose

Expresses V5-ANKRD52

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

ANKRD52 (ANKRD52 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pY013 (pcDNA3.1-hEeCpf1)

Plasmid

#69985

Purpose

Expresses humanized EeCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hEeCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MBII85-5'SS mut

Plasmid

#67631

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Mutation

mutation in 5'ss

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Mito-CaNARi

Plasmid

#64851

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean, N-terminal 30 amino acids from DAKAP1, …

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-6His-MyoX

Plasmid

#47607

Depositor

Emanuel Strehler

Article

Bennett et al Biochem Biophys Res Commun. 2008 May 2;3

Insert

MyoX (MYO10 Human)

Expression

Mammalian

Mutation

Q680R

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/GS-DJ1-L166P-V5

Plasmid

#29343

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

L166P

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-hMcl-1(S159A)

Plasmid

#25374

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1(S159A) (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed serine 159 to alanine

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 EYFP H148Q

Plasmid

#25871

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with Histidine 148 to …

Available Since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only