We narrowed to 6,690 results for: PRS

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pRS-ATG1-ATG7 3'UTR(406)

Plasmid

#122070

Purpose

Expressing ATG1 with ATG7 3'UTR

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native 3'UTR was replaced by ATG7 3'…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A)(405)

Plasmid

#122065

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(S226A,T228A)(405)

Plasmid

#122066

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Serine 226 to alanine; Threonine 228 to alanine

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)

Plasmid

#106461

Purpose

Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W

Tags

PA

Expression

Bacterial and Yeast

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)

Plasmid

#106470

Purpose

Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W

Tags

PA

Expression

Bacterial and Yeast

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

Plasmid

#68276

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4E) mCherry

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_63/pRSETB

Plasmid

#51980

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-4GS

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pRS416 Gal-RNQ1 L94A-YFP

Plasmid

#18692

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pR BirA

Plasmid

#113640

Purpose

Insect cell expression vector for BirA Biotin Ligase

Depositor

James Moon

Article

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

BirA Biotin Ligase (birA )

Expression

Insect

Promoter

Metallothionein

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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p5M-PR-A

Plasmid

#89124

Purpose

mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-A (PGR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRS-shTGFbeta1

Plasmid

#14973

Depositor

Joan Massague

Article

Thomas et al Cancer Cell. 2005 Nov . 8(5):369-80.

Insert

TGFbeta1 shRNA (Tgfb1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pRS-45

Plasmid

#40586

Depositor

Harald Schwalbe

Article

Silvers et al Protein Expr Purif. 2012 Jul 24;85(1):14

Insert

yTAND12-TEV-hTBPc (TAF1, TBP Budding Yeast, Human)

Tags

His-tag and yTAND12

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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Pr-deGFP

Plasmid

#67743

Purpose

Pr promoter expressing deGFP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pR-BAM

Plasmid

#22649

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1L (Myt1l Rat)

Use

Cloning and rna synthesis

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pR-Avr

Plasmid

#22650

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1 (Myt1 Rat)

Use

Cloning and rna synthesis

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pR-Pst

Plasmid

#22648

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1 (Myt1 Rat)

Use

Cloning and rna synthesis

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pR Leu61

Plasmid

#12230

Depositor

Sung-Hou Kim

Insert

pRas189 Leu61

Expression

Bacterial

Mutation

Gln61 to Leu61

Available Since

Sept. 18, 2006

Availability

Academic Institutions and Nonprofits only