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Showing: 1221 - 1240 of 4138 results

  1. pLyGo-Bs-1

    Plasmid
    #163137
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163137-plasmid-map-sequence-id-332146

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  2. pLyGo-Bs-3

    Plasmid
    #163141
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163141-plasmid-map-sequence-id-332141

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  3. pLyGo-Bs-2

    Plasmid
    #163140
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163140-plasmid-map-sequence-id-332139

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  4. pLyGo-Ec-3

    Plasmid
    #163129
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163129-plasmid-map-sequence-id-332101

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  5. C513-E06: attB5r-pA-polyhedrinp>-attB1r

    Plasmid
    #162948
    Purpose
    Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    AcMNPV polyhedrin promoter with upstream polyA sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162948-plasmid-map-sequence-id-330994

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  6. C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

    Plasmid
    #162965
    Purpose
    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-Halotag fusion tag with upstream E. coli ribosome binding site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    162965-plasmid-map-sequence-id-330946

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  7. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

    Plasmid
    #162964
    Purpose
    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    162964-plasmid-map-sequence-id-330937

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  11. C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r

    Plasmid
    #162934
    Purpose
    Gateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162934-plasmid-map-sequence-id-330542

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  12. C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

    Plasmid
    #162933
    Purpose
    Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-maltose binding protein fusion, AcMNPV polyhedrin promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162933-plasmid-map-sequence-id-330541

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  13. pLyGo-Ec-5

    Plasmid
    #163131
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163131-plasmid-map-sequence-id-329722

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  16. pVR2-m4

    Plasmid
    #84723
    Purpose
    pVR2 but with G to U and A to C mutations following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at the first nucleotide after the…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84723-plasmid-map-sequence-id-167501

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  17. pVR2-m5

    Plasmid
    #84724
    Purpose
    pVR2 but with an insertion of 1 U immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion of 1 U between positions 39 and 40 of a…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84724-plasmid-map-sequence-id-167505

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  18. pVR2-m6

    Plasmid
    #84725
    Purpose
    pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion of 2 Us between positions 39 and 40 of …
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84725-plasmid-map-sequence-id-167510

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  19. pVR2-m7

    Plasmid
    #84726
    Purpose
    pVR2 but with a G to U mutation immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at position 41 of annotated "…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84726-plasmid-map-sequence-id-167513

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  20. pVR2-m8

    Plasmid
    #84727
    Purpose
    pVR2 but with two G to U mutations immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutations at positions 40 and 41 of annota…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84727-plasmid-map-sequence-id-167518

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Showing: 1221 - 1240 of 4138 results