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Plasmid#136107PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptideDepositorInsertCTAG_mVenus(noATG,noStop)-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-4T Syt1 C2AB(140) M173C
Plasmid#134696PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labelingDepositorInsertSynaptotagmin 1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationM173C, C277APromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Syt1 C2AB(140) F234C
Plasmid#134697PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labelingDepositorInsertSynaptotagmin 1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationF234C, C277APromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Syt1 C2AB(140) V304C
Plasmid#134698PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labelingDepositorInsertSynaptotagmin 1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationV304C, C277APromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Syt1 C2AB(140) I367C
Plasmid#134699PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labelingDepositorInsertSynaptotagmin 1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationI367C, C277APromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid1
Plasmid#173469PurposeN-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid2
Plasmid#173470PurposeN-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid3
Plasmid#173471PurposeN-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-SAE1
Plasmid#171012PurposeEctopic expression of SAE1DepositorInsertuntagged SAE1
UseLentiviralTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-nfBFP
Plasmid#171013PurposeEctopic expression of nonfluorescent BFPDepositorInsertuntagged nfBFP
UseLentiviralTagsExpressionMammalianMutationBFP[H66A]PromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLENTI CMV GFP-TREX1 309* BLAST
Plasmid#164242Purposelentiviral plasmid for GFP-TREX1 309*DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationdeletion of amino acids 310-314PromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLz77_CB_operon_CsoS2_N3
Plasmid#140859PurposeSame as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-108 and 220-235 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz78_CB_operon_CsoS2_delNTD
Plasmid#140860PurposeSame as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)DepositorInsertCbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-235 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz172_CsoS2_N1_GFP
Plasmid#140852PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backboneDepositorInsertCsoS2 NTD repeat 1 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz175_CsoS2_N4_GFP
Plasmid#140855PurposeHis-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 4 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz173_CsoS2_N2_GFP
Plasmid#140853PurposeHis-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz174_CsoS2_N3_GFP
Plasmid#140854PurposeHis-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 3 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
Plasmid#124066PurposeE. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.DepositorInsertcytATL-R232Q-sfGFP
UseTagssfGFPExpressionBacterialMutationR232QPromotertrcAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
refGFP1_pQE30
Plasmid#48644PurposeBact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFPDepositorInsertrefGFP1
UseTags6xHisExpressionBacterialMutationrefGFP C147S, C204QPromoterAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Map7-1783-end
Plasmid#46071DepositorInsertMap7-1783-end (Map7 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only