We narrowed to 17,106 results for: grna

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lentiCRISPR - AAVS1 sgRNA

Plasmid

#70661

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against AAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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PINK1 gRNA (BRDN0001147554)

Plasmid

#78035

Purpose

3rd generation lentiviral gRNA plasmid targeting human PINK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PINK1 (PINK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pWH1266-Apra-sgRNA

Plasmid

#218372

Purpose

sgRNA expression plasmid for CRISPR-intereference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

sgRNA expression cassette

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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EPHA7 gRNA (BRDN0001145253)

Plasmid

#77351

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHA7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHA7 (EPHA7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hsf-1-sgRNA-A

Plasmid

#177786

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-2XMS2

Plasmid

#75389

Purpose

sgRNA1-2XMS2

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Sa-gRNA-puro

Plasmid

#191652

Purpose

Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

S. aureus gRNAscr

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-ABE8e_gRNA

Plasmid

#202559

Purpose

P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-eGFP

Plasmid

#116926

Purpose

Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

eGFP

Use

Retroviral

Promoter

EF1a

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-GEMSgRNA2

Plasmid

#204768

Purpose

pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

guide RNA targeting GEMS m6A sensor mRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-B

Plasmid

#177812

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU6a/LacZ

Plasmid

#66195

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6a

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pX459-Reck gRNA

Plasmid

#246546

Purpose

CRISPR vector co-expressing Cas9 and a mouse Reck gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Reck gRNA (Reck Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd1/2/4/5/7/8 gRNA

Plasmid

#246550

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2/4/5/7/8 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2/4/5/7/8 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Dvl1/3 gRNA

Plasmid

#246555

Purpose

CRISPR vector co-expressing Cas9 and a mouse Dvl1/3 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Dvl1/3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Dvl2 gRNA

Plasmid

#246556

Purpose

CRISPR vector co-expressing Cas9 and a mouse Dvl2 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Dvl2 gRNA (Dvl2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Gpr124 gRNA

Plasmid

#246547

Purpose

CRISPR vector co-expressing Cas9 and a mouse Gpr124 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Gpr124 gRNA (Adgra2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCFD1-dU6:1gRNA

Plasmid

#49408

Purpose

expression of gRNA under control of the Drosophila U6:1 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-1:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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