We narrowed to 10,428 results for: try

1. pTrex-b-NLS-hSpCas9

   Plasmid

   #62543

   Purpose

   Expression of Cas9 in T. cruzi

   Depositor

   Rick Tarleton

   Article

   Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

   Insert

   NLS-hSpCas9
   

   Use

   CRISPR; Trypanosoma cruzi expression

   Tags

   3xFLAG and NLS

   Promoter

   Ribo-HX1

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CALCOCO1 W47A-mycDDK

   Plasmid

   #137769

   Purpose

   CALCOCO1 LIR mutant

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   CALCOCO1 W47A
   

   Tags

   mycDDK

   Expression

   Mammalian

   Mutation

   W47A

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPa-iSL(MCS1+2)

   Plasmid

   #69244

   Purpose

   Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Promoter

   RRNA promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrex-Luc-Phleo

   Plasmid

   #48337

   Purpose

   Trasgenically expresses firefly luciferase in T. cruzi with phleomycin selection

   Depositor

   Rick Tarleton

   Article

   Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

   Insert

   firely luciferase
   

   Use

   Trypanasoma cruzi expression

   Promoter

   T. cruzi rRNA

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pIIS18-SapI

   Plasmid

   #128599

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIIS18-BsmBI

   Plasmid

   #128648

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET11a-DAAT-V33G/T242G

   Plasmid

   #159278

   Purpose

   Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

   Depositor

   Roberto Chica

   Article

   Parmeggiani et al ACS Catalysis 9, 3482–3486.

   Insert

   Engineered d-alanine aminotransferase
   

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Valine 33 and threonine 242 have been mutated to …

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CALCOCO1 V142A-mycDDK

   Plasmid

   #137768

   Purpose

   CALCOCO1 CLIR mutant

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   CALCOCO1 V142A
   

   Tags

   mycDDK

   Expression

   Mammalian

   Mutation

   V142A

   Promoter

   CMV

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223 LC3B2 G120

   Plasmid

   #123077

   Purpose

   Gateway entry clone encoding human MAP1LC3B2 G120

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Deleted amino acids 121-125. Stop codon after G12…

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223 LC3B2 G120A

    Plasmid

    #123076

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 G120A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 120 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL2084

    Plasmid

    #137726

    Purpose

    Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

    Depositor

    Jeremy Mottram

    Article

    Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

    Type

    Empty backbone

    Use

    RNAi; Trypanosoma brucei tet inducible stem loop

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPTI-21

    Plasmid

    #195058

    Purpose

    simplyfied amino acid sequence by alanine scanning

    Depositor

    Yutaka Kuroda, Peter Kim

    Article

    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d

    Insert

    bovine pancreatic trypsin inhibitor
    

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    21 amino acids are Alanine out of 58 amino acids

    Promoter

    T7 promotor

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPQD0CM0765

    Plasmid

    #177031

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    tryptophan decarboxylase
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIIS18-BufAI-2

    Plasmid

    #128651

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIIS18-BufAI-1

    Plasmid

    #128650

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CALCOCO1 L140A-mycDDK

    Plasmid

    #137767

    Purpose

    CALCOCO1 CLIR mutant

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    CALCOCO1 L140A
    

    Tags

    mycDDK

    Expression

    Mammalian

    Mutation

    L140A

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CALCOCO1 R12H-mycDDK

    Plasmid

    #137770

    Purpose

    CALCOCO1 R12H mutant

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    CALCOCO1 R12H
    

    Tags

    mycDDK

    Expression

    Mammalian

    Mutation

    R12H

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223 LC3B2 no-stop

    Plasmid

    #123207

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRPa-c6MYCn

    Plasmid

    #69242

    Purpose

    Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    cMyc

    Promoter

    RRNA promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRP-c6MYCn

    Plasmid

    #69243

    Purpose

    Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    cMyc

    Promoter

    RRNA promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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