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pPB-cT3G-cERP2-RB1

Plasmid

#222550

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RB1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RB1 (RB1 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HOXA10

Plasmid

#222539

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXA10

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXA10 (HOXA10 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB3

Plasmid

#222540

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB3 (HMGB3 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ESX1

Plasmid

#222563

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ESX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

ESX1 (ESX1 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1_Short_V5-GFP

Plasmid

#221500

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 224-371AA C-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 1-159AA N-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_HD_160-223AA

Plasmid

#221477

Purpose

Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1_Short_V5-GFP

Plasmid

#221498

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pWR91

Plasmid

#203915

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

CRF1 plus flanking (CRF1 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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