We narrowed to 3,663 results for: EXO

1. pEF6 rglut1 flag complete

   Plasmid

   #89571

   Purpose

   rglut1 flag complete

   Depositor

   Jeffrey Rathmell

   Article

   Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

   Insert

   rglut1 flag complete (Slc2a1 Rat)
   

   Use

   Tags

   Exo Facial

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. BUN21/pML300 (recipient strain)

   Bacterial Strain

   #11675

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Bacterial Resistance

   Spectinomycin

   Species

   Bacteriophage lambda

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pRedCmOcr

   Plasmid

   #176226

   Purpose

   A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB and PL

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY118A

   Plasmid

   #182958

   Purpose

   pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

   Depositor

   Shelley Copley

   Article

   Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

   Inserts

   Cas9
   Gam, Beta, Exo
   I-scel
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJS145

   Plasmid

   #179159

   Purpose

   Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

   Depositor

   Iva Greenwald

   Article

   Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Insert

   rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Flexon replacing 2nd intron of 2xNLS::GFP

   Promoter

   rps-27p

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SHC003BSD-DelGFP

   Plasmid

   #133301

   Purpose

   EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SHC003BSD

   Plasmid

   #133300

   Purpose

   The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TLCV2-DelGFP

   Plasmid

   #133302

   Purpose

   EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. lucMAPT-GenRep

   Plasmid

   #214674

   Purpose

   MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons

   Depositor

   Eugene Yeo

   Article

   Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   Firefly Luciferase, Renilla Luciferase, and V5

   Expression

   Mammalian

   Mutation

   Contains Exon 9 and Exon 11, with a BamHI EcoRI c…

   Promoter

   CMV

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL2-HKII-F

    Plasmid

    #64518

    Purpose

    AS-30D hepatoma hexokinase II 0.075 kbp exon I-luciferase reporter vector

    Depositor

    Peter Pedersen

    Article

    Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

    Insert

    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Isolated from AS-30D rat hepatoma

    Promoter

    Hexokinase II promoter deleted

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only