Addgene

We narrowed to 2,379 results for: LARGE

Showing: 1241 - 1260 of 2379 results

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pBAD02_CBASS_Escherichia_coli_RU1_BHI6

Plasmid

#224397

Purpose

For expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Cap15_EcCdnE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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dCBE4-gam

Plasmid

#124449

Purpose

Expresses dead Cas9 (dCas9)-CBE4-gam

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dCBE4-gam

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAF0690 Ef1a-Kp03_attB-BFP acceptor

Plasmid

#193473

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_SpikeyGi2_kv2.1

Plasmid

#195313

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_PDCD6_EF-hand

Plasmid

#110009

Purpose

Protein expression and purification of PDCD6_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PDCD6_EF-hand (PDCD6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-TSV (linearized in LT)

Plasmid

#70033

Purpose

pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region

Depositor

Mariet Feltkamp

Article

van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

Insert

TSPyV full genome, linearized in Large T-antigen

Use

Cloning vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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ires-eGFP(+HAs) donor-1

Plasmid

#87865

Purpose

Express eGFP reporter for HDR-induced knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Homologous recombination for gapdh loci

Tags

Expression

Mutation

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Bxb1_miRFP703

Plasmid

#183759

Purpose

Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

miRFP703

Use

Tags

Lck lipidation signal

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0185 Cp36_attP-mCherry donor

Plasmid

#193468

Purpose

Promoterless mcherry donor for Cp36

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36_attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_PIKFYVE_DEP

Plasmid

#109789

Purpose

Protein expression and purification of PIKFYVE_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PIKFYVE_DEP (PIKFYVE Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pASPIre1

Plasmid

#154842

Purpose

Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites

Depositor

Markus Jeschek

Article

Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

Insert

Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Prha

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pASPIre3

Plasmid

#154844

Purpose

Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

Depositor

Markus Jeschek

Article

Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

Insert

Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAD51_Rad51

Plasmid

#110031

Purpose

Protein expression and purification of RAD51_Rad51

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAD51_Rad51 (RAD51 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_Flex_syn_SpikeyGi2_kv2.1

Plasmid

#195314

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin promoter

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAF0286 Ef1a-Cp36_attB-BFP acceptor

Plasmid

#193474

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAQ_14-3-3

Plasmid

#109943

Purpose

Protein expression and purification of YWHAQ_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAQ_14-3-3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR3C1_Hormone-recep

Plasmid

#110018

Purpose

Protein expression and purification of NR3C1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR3C1_Hormone-recep (NR3C1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_S100A11_S-100

Plasmid

#109804

Purpose

Protein expression and purification of S100A11_S-100

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

S100A11_S-100 (S100A11 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAE_14-3-3

Plasmid

#109942

Purpose

Protein expression and purification of YWHAE_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAE_14-3-3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SERPINA5_Serpin

Plasmid

#110034

Purpose

Protein expression and purification of SERPINA5_Serpin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SERPINA5_Serpin (SERPINA5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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