Addgene

We narrowed to 11,264 results for: Vars

Showing: 1241 - 1260 of 11264 results

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PMKO-shNC

Plasmid

#64863

Purpose

Expresses a scrambled shRNA

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

Scrambled shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

u6

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-HCF-1-B

Plasmid

#36996

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
MAC_N_LHX4

Plasmid

#167778

Purpose

Expresses LHX4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

LHX4 (LHX4 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3xFlag-S242R-MEFV

Plasmid

#167020

Purpose

Gateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Expression

Mutation

serine 242 in arginine (S242R)

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Use

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3 humanN-Flag SadBS

Plasmid

#66881

Purpose

Mammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pBW105

Plasmid

#111137

Purpose

For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

Depositor

Alexander Varshavsky

Article

Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

Insert

MLS-ratAANAT-3FLAG (Aanat Rat)

Use

Tags

3x Flag

Expression

Yeast

Mutation

Promoter

Gal1

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pmCellFree _KA2_ZIKV NS5

Plasmid

#145368

Purpose

Cell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-RHEB-IRES-Puro

Plasmid

#120576

Purpose

Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGex2t SadBs

Plasmid

#66882

Purpose

Bacterial expression vector encoding N-terminally GST-tagged short isoform of human SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pNB695

Plasmid

#60144

Purpose

Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pNB697

Plasmid

#60146

Purpose

Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe hygro NFS1 CD

Plasmid

#102978

Purpose

Expression of NFS1 C381A mutant

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 C381A (NFS1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C381A catalytic site mutant, Silent mutations to …

Promoter

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
pNB788

Plasmid

#60165

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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Tensin-2 Y483E

Plasmid

#201794

Purpose

Expresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Use

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

mutation Y483E

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Tensin-2 Y483F

Plasmid

#201795

Purpose

Expresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Use

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

mutation Y483F

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Tensin-2 Y483A

Plasmid

#201796

Purpose

Expresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Use

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

mutation Y483A

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-CDK8

Plasmid

#222609

Purpose

Expression of MAC3-tagged CDK8

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

CDK8 (CDK8 Human)

Use

Tags

MAC3

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mCh-Miro1(∆TM)-SspB

Plasmid

#194007

Purpose

Optogenetic tool

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_C_FLT1

Plasmid

#187784

Purpose

MAC-tagged gene expression of human FLT1

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

FLT1 (FLT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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