We narrowed to 5,553 results for: golden gate
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Plasmid#209389PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneTagsGFPExpressionPlantMutationTurboID gene sequence C249AAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only
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Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRExpressionBacterialAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2215 - Intron GG3 - 900 bp
Plasmid#159882PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG3 - 900 bp
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC71171
Plasmid#154069PurposeLevel 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backboneDepositorInsertCRE_U5intron
UseSynthetic BiologyMutationAll BsaI, Esp3I and BPiI restriction sites were r…Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-term tag YFP
UseSynthetic BiologyMutationG65S, L68V, A72S, Y203T, L231HAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GreenGate 2.0 Toolkit
Plasmid kit#1000000241PurposeCompatible with GreenGate Cloning System (#1000000036). Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and fluorescent plasma membrane and nuclear markers.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector typePlant ExpressionEditing typeCRISPRCloning typeGolden Gate (GreenGate)Available sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PBgg-Puro
Plasmid#248967PurposeGolden Gate assembly-friendly PiggyBac backbone with Puromycin resistanceDepositorTypeEmpty backboneExpressionMammalianAvailable sinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
GGDest2-Amp
Plasmid#157650PurposeGolden Gate assembly of a pET28-compatible fusion-protein ORF insertDepositorTypeEmpty backboneUseSynthetic BiologyPromoternoneAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
GGDest3-Amp
Plasmid#157651PurposeGolden Gate assembly of a pTXB1 compatible fusion-protein ORF insertDepositorTypeEmpty backboneUseSynthetic BiologyPromoternoneAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB90 - pAGM4723_VirGN54D
Plasmid#123187PurposeGolden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in AgrobacteriaDepositorTypeEmpty backboneUseLevel 2 acceptor vector for expression in plantsExpressionPlantMutationVirGN54D for increase virulenceAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJEC819
Plasmid#190815PurposeCRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-XTEN-rpoA
UseCRISPR and Synthetic BiologyTagsXTEN (linker), followed by the N-terminal domain …PromoterSP30Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA
Plasmid#179211PurposeGolden Gate entry vector; 2nd gRNA with tRNADepositorTypeEmpty backboneUseCRISPRPromoterno promoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-T3G-ERP2
Plasmid#175188PurposeAll-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3GAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ138A
Plasmid#69280PurposeGolden Gate entry vector; 8th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ137A
Plasmid#69279PurposeGolden Gate entry vector;7th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ147
Plasmid#69299PurposeGolden Gate recipient and Gateway entry vector; assembly of 7 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
L0-I18 Ub-Arg GG1-GG2
Plasmid#195899PurposeLevel 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkersDepositorInsertUb-Arg tag
UseSynthetic BiologyAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAP1_Ta_Yr7_CDS_no_STOP
Plasmid#141093PurposeLevel 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codonDepositorInsertTa_Yr7
UseUnspecified; Level 0 module for golden gate cloni…Available sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132D-RZ-Lb
Plasmid#134348PurposeGolden Gate entry vector; empty vector to clone the 2nd LbCas12a crRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFUSC9
Plasmid#50429PurposePlasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 9.5 RVDs long.DepositorPublicationTypeEmpty backboneAvailable sinceJan. 29, 2014AvailabilityAcademic Institutions and Nonprofits only