We narrowed to 5,224 results for: golden gate

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SM426_pHAGE-CAG-mCherry-T2A-puro-DR-As_crRNA-KAN-DR

Plasmid

#244226

Purpose

Vector for golden gate cloning AsCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1058

Plasmid

#209548

Purpose

BTK Type 1 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Upstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1052

Plasmid

#209542

Purpose

BTK Type 2 antibiotic resistance cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Type 2 Kanamycin resistance cassette

Use

Synthetic Biology

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1053

Plasmid

#209543

Purpose

BTK Type 3-4 fluorescent protein cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Type 3-4 E2-Crimson cassette

Use

Synthetic Biology

Promoter

CP25

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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SL3-MS2-gRNA GG backbone (MOBE3-4)

Plasmid

#219949

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-SL3 (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK1059

Plasmid

#209549

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Downstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Available Since

Feb. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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L0-I2 pPP2AA3-mutated GG0-GG1

Plasmid

#195891

Purpose

Level 0 pPP2AA3 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3

Use

Synthetic Biology

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNL2114 pARF19 GG0-GG1

Plasmid

#195909

Purpose

Level 0 pARF19 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19 (ARF19 Mustard Weed)

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I23 Bxb1 GG1-GG4

Plasmid

#195901

Purpose

Level 0 Bxb1 integrase with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Bxb1 integrase

Use

Synthetic Biology

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I10 PhiC31 GG2-GG4

Plasmid

#195893

Purpose

Level 0 PhiC31 integrase with GG2 GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I11 PhiC31-NLS GG1-GG4

Plasmid

#195894

Purpose

Level 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I16 tUBQ1 GG5-GG6

Plasmid

#195898

Purpose

Level 0 tUBQ1 terminator with GG5 GG6 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

tUBQ1 (UBQ1 Mustard Weed)

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I12 PhiC31-NLS GG2-GG4

Plasmid

#195895

Purpose

Level 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I15 tUBQ1 GG4-GG6

Plasmid

#195897

Purpose

Level 0 tUBQ1 terminator with GG4 GG6 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

tUBQ1 (UBQ1 Mustard Weed)

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I26 pUBQ10 GG0-GG1

Plasmid

#195902

Purpose

Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10 (UBQ10 Mustard Weed)

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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GGDest1-Amp

Plasmid

#157649

Purpose

Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL12022

Plasmid

#117510

Purpose

AtAG promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pAG:AATG:BsaI

Expression

Plant

Promoter

AtAG

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJEC726

Plasmid

#190814

Purpose

CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

Depositor

James Chappell

Article

Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

dCas9

Use

CRISPR and Synthetic Biology

Mutation

S. pyogenes dCas9 codon optimized for Streptomyce…

Promoter

SP30

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ133-tRNA

Plasmid

#179212

Purpose

Golden Gate entry vector; 3rd gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Promoter

no promoter

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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