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We narrowed to 4,072 results for: golden gate

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Showing: 1241 - 1260 of 4072 results

  1. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  2. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  3. P57m

    Plasmid
    #120949
    Purpose
    MoClo golden gate assembly AB part for pCin* (Adam Meyer's improved pCin promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pCin Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120949-plasmid-map-sequence-id-235961

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  4. pNDMS239

    Plasmid
    #248096
    Purpose
    mScarlet-I fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    mScarlet-I
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248096-plasmid-map-sequence-id-495375

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  5. RcP9_AraC_S170_LOV_P190_Sil_90bp

    Plasmid
    #242158
    Purpose
    Recombineering Plasmid 9. Positive control to test for successful editing using AraC S170 LOV (silent mutation of P190, 90bp retron)
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242158-plasmid-map-sequence-id-492364

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  6. pKS1604

    Plasmid
    #239528
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    239528-plasmid-map-sequence-id-487894

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  7. pKS1606

    Plasmid
    #239529
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    239529-plasmid-map-sequence-id-487896

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  8. pDest_PANCEd_AraC_S170_LOV_sd2_WT

    Plasmid
    #242151
    Purpose
    Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170
    Depositor
    Dominik Niopek
    Insert
    AraC S170 LOV
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242151-plasmid-map-sequence-id-484656

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  9. pVL1173

    Plasmid
    #239536
    Purpose
    Express CpPT1 membrane protein cargo with N-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239536-plasmid-map-sequence-id-479033

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  10. pN4

    Plasmid
    #233521
    Purpose
    Nb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARS
    Depositor
    Carl Denard
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Promoter
    pGAL1-10
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233521-plasmid-map-sequence-id-471179

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  11. pKS1620

    Plasmid
    #239551
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239551-plasmid-map-sequence-id-478368

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  12. pKS1617

    Plasmid
    #239548
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239548-plasmid-map-sequence-id-478367

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  13. pKS1618

    Plasmid
    #239549
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239549-plasmid-map-sequence-id-478366

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  14. pVL1172

    Plasmid
    #239535
    Purpose
    Express CpPT1 membrane protein cargo with C-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-mTurquoise2
    Expression
    Yeast
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239535-plasmid-map-sequence-id-476352

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  15. pKS1619

    Plasmid
    #239550
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239550-plasmid-map-sequence-id-475997

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  16. pKS1601

    Plasmid
    #239526
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239526-plasmid-map-sequence-id-475905

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  17. pKS1531

    Plasmid
    #239521
    Purpose
    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239521-plasmid-map-sequence-id-475896

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  18. pKS1575

    Plasmid
    #239523
    Purpose
    Express Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScVba2p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239523-plasmid-map-sequence-id-475887

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  19. pVL1337

    Plasmid
    #239541
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex15 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-ScPex15(315-383)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239541-plasmid-map-sequence-id-475879

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  20. pKS1574

    Plasmid
    #239522
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScYea4p-mTurquoise2
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239522-plasmid-map-sequence-id-475877

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Showing: 1241 - 1260 of 4072 results