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Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pRL093 pattB_exorh:EGFP_MCS
Plasmid#213514PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1-donor
Plasmid#112295PurposeCRISPR donor plasmid to tag human transcription factor NR4A1 with GFPDepositorInsertNR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
UseCRISPRTagsExpressionMutationhg38:12:52058840:C>T, hg38:12:52058930:A>C,…PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL092 pattB_cryaa:Venus_MCS
Plasmid#213513PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-tdTomato-polyA
Plasmid#174022PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserttdTomato
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOTag-glmS-4H
Plasmid#106378PurposeCan be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulationDepositorInsertGlmS-3xHA
UseTagsExpressionMutationPromoterAvailable sinceMarch 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-FKBP-GFP-HIS3MX6
Plasmid#183023PurposePCR-based tagging of yeastDepositorInsertFKBP
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Monomer NH_V2
Plasmid#37527Purposetemplate for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE.DepositorInsertMonomer NH
UseTagsExpressionMutationPromoterAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEP-EGFPin
Plasmid#60961PurposePCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesisDepositorInserteGFP-Kana_I-SceI
UseTagsExpressionMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-mLanYFP-polyA
Plasmid#174021PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertmLanYFP
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECT3
Plasmid#75450PurposePlasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt3
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA CUP1p-NuiHA kanMX
Plasmid#131169PurposePCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneUseTagsNui-HAExpressionYeastMutationPromoterAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
punc-119cR
Plasmid#21596DepositorInsertunc-119:mCherry (unc-119 Nematode)
UseAdds unc-119 marker to amp resistant plasmidsTagsmCherryExpressionWormMutationN/APromoterAvailable sinceAug. 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCfB8742
Plasmid#126908PurposeTemplate for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolyticaDepositorInsertpGPD1 and pFBA1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECT5
Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECM1
Plasmid#75445PurposePlasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
U6>XX
Plasmid#87542PurposeComponent 1 of 2 for U6>sgRNA cassette OSO-PCRDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL2080 pGATA23 GG0-GG1
Plasmid#195908PurposeLevel 0 pGATA23 promoter with GG0 and GG1 golden gate linkersDepositorInsertpGATA23
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUNB-donor
Plasmid#112313PurposeCRISPR donor plasmid to tag human transcription factor JUNB with GFPDepositorInsertJUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
UseCRISPRTagsExpressionMutationhg38:19:12792992:C>G, hg38:19:12793018:T>G,…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only