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Showing: 1891 - 1920 of 7088 results

  4. MCP-3x9aaTAD

    Plasmid
    #210707
    Purpose
    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210707-plasmid-map-sequence-id-416252

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  6. PB-iRFP-STOP-ReNL

    Plasmid
    #113965
    Purpose
    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Inserts
    iRFP670
    ReNL
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV - SV40-PolyA - SV40-PolyA and EF1A
    Available since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    113965-plasmid-map-sequence-id-223037

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  8. pCKC775

    Plasmid
    #226626
    Purpose
    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    7xTCF-LEF and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226626-plasmid-map-sequence-id-449161

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  9. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, S. pyogenes, Human)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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  10. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Mutation
    Promoter
    4xHRE_YB TATA and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  12. pRS191

    Plasmid
    #197140
    Purpose
    scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)
    Depositor
    Nozomu Yachie
    Article
    Ishiguro et al bioRxiv 2023.01.18.524633
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EGFP(M1V)
    Promoter
    EF1a
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197140-plasmid-map-sequence-id-392613

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  13. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

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  14. piggyBAC-U6-GFP-WPRE

    Plasmid
    #200030
    Purpose
    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200030-plasmid-map-sequence-id-393204

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  15. pDY0551-Luciferase RADARS

    Plasmid
    #182538
    Purpose
    Mammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Insert
    RADARS Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182538-plasmid-map-sequence-id-356645

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  16. ER-P-TAG (pGS594)

    Plasmid
    #160039
    Purpose
    pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Insert
    CP450TMD-APEX2-3xHA
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160039-plasmid-map-sequence-id-327043

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  17. lenti-RfxCas13d-Blast-BFP

    Plasmid
    #196726
    Purpose
    Sortable and selectable RfxCas13d.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196726-plasmid-map-sequence-id-389737

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  18. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

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  19. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

    Plasmid
    #219828
    Purpose
    Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 31, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    219828-plasmid-map-sequence-id-441125

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  20. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

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  21. pLV Rnf121-V5

    Plasmid
    #175130
    Purpose
    Lentiviral expression of mouse Rnf121-V5
    Depositor
    Larry Gerace
    Insert
    Rnf121 (Rnf121 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175130-plasmid-map-sequence-id-352014

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  22. pLV Nup62-V5

    Plasmid
    #175116
    Purpose
    Lentiviral expression of mouse Nup62-V5
    Depositor
    Larry Gerace
    Insert
    Nup62 (Nup62 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175116-plasmid-map-sequence-id-351797

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  23. pLV Tor1a-V5

    Plasmid
    #175135
    Purpose
    Lentiviral expression of Tor1a-Myc
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175135-plasmid-map-sequence-id-352022

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  24. pLV V5-Mospd1

    Plasmid
    #175132
    Purpose
    Lentiviral expression of V5-tagged mouse Mospd1
    Depositor
    Larry Gerace
    Insert
    Mospd1 (Mospd1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175132-plasmid-map-sequence-id-352019

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  25. pLV Tyw1-V5

    Plasmid
    #175131
    Purpose
    Lentiviral expression of mouse Tyw1-V5
    Depositor
    Larry Gerace
    Insert
    Tyw1 (Tyw1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175131-plasmid-map-sequence-id-352017

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  27. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid
    #229498
    Purpose
    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.
    Depositor
    Davide Ruggero
    Article
    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/
    Insert
    MYC 5'Untranslated region + destabilized GFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229498-plasmid-map-sequence-id-456853

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  28. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Antonella Fidanza, Lesley Forrester
    Article
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211495-plasmid-map-sequence-id-454077

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  30. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    92065-plasmid-map-sequence-id-181210

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Showing: 1891 - 1920 of 7088 results