We narrowed to 165,694 results for: addgene

1. pEN_TT DNM1L-V5

   Plasmid

   #220359

   Purpose

   Gateway entry vector for an inducible V5-tagged DNM1L

   Depositor

   Kevin Janes

   Insert

   DNM1L (DNM1L Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   V5

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

   Plasmid

   #185061

   Purpose

   Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1-GFP_mmu-miR-292b-3p_sponge

   Plasmid

   #132622

   Purpose

   Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV V5-Eva1b

   Plasmid

   #175158

   Purpose

   Lentiviral expression of V5-tagged mouse Eva1b

   Depositor

   Larry Gerace

   Insert

   Eva1b (Eva1b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV Cenpv-V5

   Plasmid

   #175125

   Purpose

   Lentiviral expression of mouse Cenpv-V5

   Depositor

   Larry Gerace

   Insert

   Cenpv (Cenpv Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. XLone-BSD NFIL3 T2A Puro

   Plasmid

   #140028

   Purpose

   Tunable and temporal expression control of NFIL3 and puro resistant gene

   Depositor

   Xiaoping Bao

   Insert

   NFIL3 (NFIL3 Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   puromycin

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

   Plasmid

   #159919

   Purpose

   Mutagenesis of Vamp1

   Depositor

   Larry Zweifel

   Insert

   Vamp1 (Vamp1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pARID4B.1.0-gDNA

   Plasmid

   #132436

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ARID4B (ARID4B Human)
   

   Use

   CRISPR

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDK365

   Plasmid

   #134195

   Purpose

   Retroviral plasmid with SKAP insert

   Depositor

   Iain Cheeseman

   Insert

   SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)
   

   Use

   Retroviral

   Tags

   LAP (GFP)

   Expression

   Mammalian

   Mutation

   Alternate Start, G5 siRNA hardened

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSRNP3.1.0-gDNA

    Plasmid

    #132472

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CSRNP3 (CSRNP3 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHOXB6.1.0-gDNA

    Plasmid

    #132458

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGTF2I.1.0-gDNA

    Plasmid

    #132452

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    GTF2I (GTF2I Human)
    

    Use

    CRISPR

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHMG20B.1.0-gDNA

    Plasmid

    #132449

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    HMG20B (HMG20B Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMECOM.1.0-gDNA

    Plasmid

    #132445

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    MECOM (MECOM Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLF10.1.0-gDNA

    Plasmid

    #132433

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    KLF10 (KLF10 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBbi-Pur-AktAR2-D

    Plasmid

    #125196

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    AktAR2-D
    

    Use

    Transposon

    Tags

    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSX2.1.0-gDNA

    Plasmid

    #113793

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MSX2

    Depositor

    Kevin White

    Insert

    MSX2 (MSX2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTOX2.1.0-gDNA

    Plasmid

    #113787

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TOX2

    Depositor

    Kevin White

    Insert

    TOX2 (TOX2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTSHZ2.1.0-gDNA

    Plasmid

    #113778

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TSHZ2

    Depositor

    Kevin White

    Insert

    TSHZ2 (TSHZ2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTCFL5.1.0-gDNA

    Plasmid

    #113775

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TCFL5

    Depositor

    Kevin White

    Insert

    TCFL5 (TCFL5 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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