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We narrowed to 3,637 results for: golden gate

Showing: 1261 - 1280 of 3637 results

  1. pPD708 DsRed-Express2-ZF21

    Plasmid
    #138795
    Purpose
    Constitutive expression of DsRed-Express2-ZF21 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF21
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138795-plasmid-map-sequence-id-320733

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  2. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

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  3. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Richard Murray
    Insert
    RhlR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120969-plasmid-map-sequence-id-238056

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  4. pPD706 DsRed-Express2-ZF13

    Plasmid
    #138793
    Purpose
    Constitutive expression of DsRed-Express2-ZF13 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF13
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138793-plasmid-map-sequence-id-320376

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  5. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  6. C71m

    Plasmid
    #120983
    Purpose
    MoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    CinR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120983-plasmid-map-sequence-id-236042

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  7. pVL1710

    Plasmid
    #239543
    Purpose
    Express Yea4p membrane protein cargo fused to C-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScYea4p-2xCrk_SH3-mTurq2-TEVsite-Venus
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239543-plasmid-map-sequence-id-476623

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  8. pVL1708

    Plasmid
    #239542
    Purpose
    Express Yea4p membrane protein cargo fused to N-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    Venus-TEVsite-ScYea4p-mTurq2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239542-plasmid-map-sequence-id-476621

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  10. pKS1612

    Plasmid
    #239530
    Purpose
    Express CpPT1 membrane protein cargo without mTurqoise2 marker fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-SKL
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239530-plasmid-map-sequence-id-475878

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  11. pVL1279

    Plasmid
    #239538
    Purpose
    Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239538-plasmid-map-sequence-id-475862

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  12. pAIP4_1YW5

    Plasmid
    #234235
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1YW5 (ESS1 Candida albicans)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234235-plasmid-map-sequence-id-464199

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  13. pAIP4_4HS5

    Plasmid
    #234223
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4HS5
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234223-plasmid-map-sequence-id-464188

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  14. pAIP4_1UKF

    Plasmid
    #234214
    Purpose
    Heterologous protein expression of cysteine protease avirulence protein AvrPphB in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UKF
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234214-plasmid-map-sequence-id-464179

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  15. pAIP4_1LU4

    Plasmid
    #234207
    Purpose
    Heterologous protein expression of soluble secreted antigen MPT53 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1LU4 (mpt53 Mycobacterium tuberculosis)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234207-plasmid-map-sequence-id-464172

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  16. pAIP4_1KAF

    Plasmid
    #234209
    Purpose
    Heterologous protein expression of middle transcription regulatory protein motA in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1KAF (motA Tequatrovirus T4)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234209-plasmid-map-sequence-id-464174

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  17. pAIP4_1TJE

    Plasmid
    #234203
    Purpose
    Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TJE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234203-plasmid-map-sequence-id-464168

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  18. pAIP4_1TP9

    Plasmid
    #234200
    Purpose
    Heterologous protein expression of peroxiredoxin-2 (Prx) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TP9 (LOC7476296 Populus trichocarpa)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234200-plasmid-map-sequence-id-464165

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  19. pAIP4_5H28

    Plasmid
    #234184
    Purpose
    Heterologous protein expression of oxysterol-binding protein homolog 1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5H28 (SWH1 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234184-plasmid-map-sequence-id-464149

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  20. pAIP4_4RZ9

    Plasmid
    #234164
    Purpose
    Heterologous protein expression of pre-mRNA-splicing factor 38A in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4RZ9 (PRPF38A Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234164-plasmid-map-sequence-id-464130

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Showing: 1261 - 1280 of 3637 results