Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,072 results for: golden gate

Clear keywords
Showing: 1261 - 1280 of 4072 results

  1. pAIP4_3W4Q

    Plasmid
    #234236
    Purpose
    Heterologous protein expression of beta-lactamase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3W4Q
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234236-plasmid-map-sequence-id-464200

    Enlarge

  2. pAIP4_3ILS

    Plasmid
    #234215
    Purpose
    Heterologous protein expression of norsolorinic acid synthase (NSAS) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ILS
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234215-plasmid-map-sequence-id-464180

    Enlarge

  3. pAIP4_5NJO

    Plasmid
    #234202
    Purpose
    Heterologous protein expression of putative cupin_2 domain-containing isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5NJO
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234202-plasmid-map-sequence-id-464167

    Enlarge

  4. pAIP4_1ZHV

    Plasmid
    #234197
    Purpose
    Heterologous protein expression of aspartate kinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1ZHV
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234197-plasmid-map-sequence-id-464162

    Enlarge

  5. pAIP4_1NG6

    Plasmid
    #234191
    Purpose
    Heterologous protein expression of YqeY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1NG6 (yqeY Bacillus subtilis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234191-plasmid-map-sequence-id-464156

    Enlarge

  6. pAIP4_4QB4

    Plasmid
    #234187
    Purpose
    Heterologous protein expression of thermonuclease (TNase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QB4
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234187-plasmid-map-sequence-id-464152

    Enlarge

  7. pAIP4_2OHW

    Plasmid
    #234178
    Purpose
    Heterologous protein expression of YueI in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OHW (yueI Bacillus subtilis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234178-plasmid-map-sequence-id-464143

    Enlarge

  8. pAIP4_4CFI

    Plasmid
    #234163
    Purpose
    Heterologous protein expression of flagellin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4CFI (QUF21_RS17235 Burkholderia pseudomallei K96243)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234163-plasmid-map-sequence-id-464129

    Enlarge

  9. L1P139 - pSPCH-PhiC31-tUBQ1

    Plasmid
    #219703
    Purpose
    Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH:PhiC31:tUBQ1
    Expression
    Plant
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219703-plasmid-map-sequence-id-435733

    Enlarge

  10. L0-T15 BpiI-p35s-BpiI

    Plasmid
    #219698
    Purpose
    Level 0 to insert p35S promoter to history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    BpiI-p35s-BpiI
    Use
    Synthetic Biology
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219698-plasmid-map-sequence-id-435729

    Enlarge

  11. pBD026

    Plasmid
    #201309
    Purpose
    AvrSr27-2 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-2
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201309-plasmid-map-sequence-id-410982

    Enlarge

  12. pBD025

    Plasmid
    #201308
    Purpose
    AvrSr27-1 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-1
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201308-plasmid-map-sequence-id-409510

    Enlarge

  13. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

    Enlarge

  14. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

    Enlarge

  15. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

    Enlarge

  16. U19m

    Plasmid
    #121024
    Purpose
    MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121024-plasmid-map-sequence-id-238066

    Enlarge

  17. T15m

    Plasmid
    #121034
    Purpose
    MoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S3P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121034-plasmid-map-sequence-id-245426

    Enlarge

  18. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

    Enlarge

  19. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

    Enlarge

  20. C82m

    Plasmid
    #120993
    Purpose
    MoClo golden gate assembly CD part for RpaI (synthase for Rpa-detected quorum sensing molecule 4-coumaroyl-homoserine lactone). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120993-plasmid-map-sequence-id-236098

    Enlarge

Showing: 1261 - 1280 of 4072 results