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pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

Plasmid

#31483

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro

Use

Lentiviral

Mutation

Traffic Light 2.0 series have a +3 PEST sequence …

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSAM_Bt

Plasmid

#112497

Purpose

Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

Depositor

Andrew Goodman, Jeffrey Gordon

Article

Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

Inserts

INSeq-adapted mariner transposon
Himar1C9

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP3K7

Plasmid

#23693

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K7 (MAP3K7 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pMDIAI

Plasmid

#51655

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

apramycin antibiotic resistance gene

Use

Genetic modification

Promoter

unknown

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pX330 spCas9-mSA

Plasmid

#113096

Purpose

pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

Synthetic Biology

Tags

MSA (monomeric streptavidin

Mutation

none

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pShuttle mCherry-Tubulin

Plasmid

#26768

Depositor

Torsten Wittmann

Article

Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
hGFAP-fLuc

Plasmid

#40589

Depositor

Albee Messing

Article

Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

Insert

hGFAP promoter (GFAP Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Contains human GFAP promoter fragment (-2163 to +…

Promoter

GFAP promoter

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pRSETa_ mEos4b

Plasmid

#51073

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

Depositor

Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4b

Expression

Bacterial

Promoter

T7

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-PTK2

Plasmid

#23902

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK2 (PTK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

Plasmid

#162280

Purpose

Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN8A Variant 1, stabilized (SCN8A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

Modified human HSPA5 intron 4 inserted after bp23…

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-TBK1

Plasmid

#23851

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TBK1 (TBK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pF150 pSOD1G93AAcGFP1

Plasmid

#26411

Purpose

contains G94A mutation in pSOD1

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G94A

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D770-N771 insNPG

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Insert

Src biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLI50

Plasmid

#13573

Depositor

Chia Lee

Article

Lee et al Gene. 1991 Jul 15. 103(1):101-5.

Type

Empty backbone

Use

S. aureus-e. coli shuttle vector

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pSBbi-GN

Plasmid

#60517

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSBbi-GB

Plasmid

#60520

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only