Addgene

We narrowed to 8,161 results for: PAC;

Showing: 1281 - 1300 of 8161 results

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FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

Plasmid

#58438

Purpose

Expresses LexA-ER-Gal4AD from yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-Gal4AD

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAC2-dual-dCas9VP48-sgExpression

Plasmid

#48236

Purpose

Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pAC153-dual-dCas9VP96-sgExpression

Plasmid

#48239

Purpose

Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP96

Expression

Mammalian

Mutation

D10A H840A

Promoter

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pACT1:Cas9-GFP, U6:sgTK

Plasmid

#122852

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgTK

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Expression

Mutation

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-pac-T2A-FLPo

Plasmid

#124835

Purpose

Puromycin resistance gene and FLP recombinase

Depositor

Mario Capecchi

Insert

pac-T2A-FLPo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pF142 pAcGFP1 SOD1 A4V

Plasmid

#26403

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

A4V

Promoter

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pNatA (pACYCduet-naa10-naa15)

Plasmid

#72928

Purpose

Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

naa10 (naa10 Synthetic, Fission Yeast)
naa15 (naa15 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Codon optomised for E.coli expression

Promoter

T7

Available since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR-Kv2.1PR

Plasmid

#195525

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pPyPGK-EGFP-TM-IRES-Pac

Plasmid

#183604

Purpose

Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

Human PDGFRB transmembrane domain and Mouse IgGK …

Expression

Mammalian

Mutation

Promoter

Mouse Pgk1

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-RLuc-A90-HhR_O

Plasmid

#147073

Purpose

Insect Expression of A90-HhR

Depositor

Elisa Izaurralde

Insert

A90-HhR

Use

Tags

Expression

Insect

Mutation

A127 instead of A90 (see comments below)

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-CR7T-gRNA2.1-nlsBFP

Plasmid

#170515

Purpose

gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

CR7T

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA5-PSMA6-PSMA7

Plasmid

#217894

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMB5-PSMB6-PSMB7

Plasmid

#217898

Purpose

Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMB5 (PSMB5 Human)
PSMB6 (PSMB6 Human)
PSMB7 (PSMB7 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

Plasmid

#181956

Purpose

Genetically encoded FRET-based cAMP indicator fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)

Use

Tags

AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPyCAG-EGFP-TM-IRES-Pac

Plasmid

#183603

Purpose

Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

Human PDGFRB transmembrane domain and Mouse IgGK …

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKJ1267 (aka pAC-alphaGal4)

Plasmid

#21869

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase-SAT1

Plasmid

#162557

Purpose

For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Use

Tags

Expression

Yeast

Mutation

Promoter

Actine

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAC8_MF-p10-mCh (VE5626)

Plasmid

#139772

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

mCherry fluorescent protein

Use

Tags

Expression

Mutation

Promoter

PH

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-FLuc-Stop-5BoxB

Plasmid

#21301

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

5BoxB

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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