We narrowed to 2,475 results for: T2A

1. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

   Plasmid

   #86457

   Purpose

   Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

   Depositor

   Ralf Kuehn

   Article

   Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   BFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Pgk

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

   Plasmid

   #166693

   Purpose

   Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9-VPR
   3x Cnga1 promoter-targeting sgRNAs
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   TRE and U6

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

   Plasmid

   #195548

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

   Plasmid

   #239974

   Purpose

   PiggyBac plasmid for inducible PEmax expression and Puromycin selection

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc

   Plasmid

   #193346

   Purpose

   tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

   Plasmid

   #187334

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE

   Plasmid

   #161925

   Purpose

   Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pEF

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

   Plasmid

   #195549

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

   Plasmid

   #194279

   Purpose

   Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

   Inserts

   humanized VP64 dSaCas9 VP64 T2A Thy1.1
   gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hU6 and hUbC

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRCA892 - TFORF Empty Vector Puro-T2A-GFP (pRCA886 backbone)

    Plasmid

    #238180

    Purpose

    Lentiviral empty vector

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid

    #66708

    Purpose

    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

    Depositor

    Charles Gersbach

    Insert

    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187337

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid

    #167971

    Purpose

    Tp63 reporter targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    Tp63 (TP63 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc

    Plasmid

    #193347

    Purpose

    tetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2, Pou5f1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Sox2A61V is a highly cooperative point mutant of …

    Promoter

    TetO mini CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

    Plasmid

    #162486

    Purpose

    For generating transgenic mice expressing ERK biosensor and RGECO1

    Depositor

    Michiyuki Matsuda

    Article

    Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

    Insert

    ERK biosensor based on FRET, and RGECO1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid

    #172915

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7f-T2A-tdTomato
    

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

    Plasmid

    #217305

    Purpose

    sgRNA BFP + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-BFP
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    U6, Ef1alpha

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid

    #167943

    Purpose

    SFTPC reporter targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SFTPC (SFTPC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    EF1a

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo

    Plasmid

    #163084

    Purpose

    rTetR DNA binding domain fused to GBP (anti-GFP nanobody)

    Depositor

    Lacra Bintu

    Article

    Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

    Insert

    anti-GFP nanobody
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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