Addgene

We narrowed to 26,848 results for: Tat

Showing: 1281 - 1300 of 26848 results

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pLV-SI-112

Plasmid

#131127

Purpose

C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Mutant EGFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX5X.1 PINK1 KD

Plasmid

#13322

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496; Kinase dead: K219D/D362A/D3…

Promoter

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pLKO-puro-sh-mFAK B

Plasmid

#37018

Depositor

Bob Weinberg

Article

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

sh-mFAK B (Ptk2 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pCEP4-BaLgp160-754*

Plasmid

#123212

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEM09

Plasmid

#135479

Purpose

Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Use

Tags

GFP and hph

Expression

Bacterial and Yeast

Mutation

Promoter

Spun Hsp70pr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172670

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

SDHB pegRNA and SDHB_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Use

Tags

Expression

Mutation

Promoter

tight TRE

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRVdG-4RFP

Plasmid

#52489

Purpose

Expresses TagRFP-T

Depositor

Sebastian Seung

Article

Nakashiba et al Cell. 2012 Mar 30;149(1):188-201. doi: 1

Insert

TagRFP-T

Use

Rabies virus

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSI-535

Plasmid

#131130

Purpose

gRNA expression vector for C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

C-to-T reporter activation gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-LOx-IRES-tdTomato

Plasmid

#166919

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-hM4D(Gi)-mCherry

Plasmid

#190240

Purpose

Expresses iDREADD fused to mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

mouse CamKII alpha

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4L-Erk1

Plasmid

#39225

Depositor

Melanie Cobb

Article

Frost et al Proc Natl Acad Sci U S A. 1994 Apr 26;91

Insert

Erk1 (MAPK3 Human)

Use

Episomal

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pCEP4-ZM197M-gp160

Plasmid

#123226

Purpose

Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (ZM197M) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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GAL4-mNFAT1(2-144)

Plasmid

#11108

Purpose

Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

Depositor

Anjana Rao

Article

Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

Insert

NFAT1 (Nfatc2 Mouse)

Use

Tags

GAL4-DBD

Expression

Mammalian

Mutation

Contains NFAT1 amino acids 2-144; L78P and P140Q …

Promoter

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-Q769gp160

Plasmid

#100928

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-MA-VN

Plasmid

#123283

Purpose

Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

Matrix

Use

Tags

VN: N-terminus of split Venus (a.a. V1–A154) I152…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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SMT205

Plasmid

#134817

Purpose

SMT201 with mutated RBS for DHFR, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al Elife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Use

Tags

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-SHIV327C-HuA10-gp160

Plasmid

#123270

Purpose

Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

SHIV327C-Hu A10 Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-HGM (optimized)

Plasmid

#220988

Purpose

Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

HaloTag-GFP-mito

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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