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Showing: 1281 - 1300 of 1648 results

  10. pET23a-H6-TEV-AncSZ

    Plasmid
    #214235
    Purpose
    Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation
    Depositor
    Neel Shah
    Article
    Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:
    Insert
    Syk kinase (SYK Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence gained through ancestral reconstruction …
    Promoter
    T7
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214235-plasmid-map-sequence-id-422817

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  11. pRH3175

    Plasmid
    #210177
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    R33G,G141S
    Promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210177-plasmid-map-sequence-id-415795

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  13. pET24a-LaM4-2xTS

    Plasmid
    #162779
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162779-plasmid-map-sequence-id-321884

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  14. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid
    #140454
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, tyrosine 616 to methioni…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140454-plasmid-map-sequence-id-292302

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  15. pHR-BTLA (PD-1 ITIM)-mGFP

    Plasmid
    #180818
    Purpose
    Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP
    Depositor
    Enfu Hui
    Article
    Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1
    Insert
    BTLA (PD-1 ITIM) (BTLA Human)
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mutation
    replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…
    Promoter
    SFFV
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    180818-plasmid-map-sequence-id-356696

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  18. LIC-Z-Y-L

    Plasmid
    #153546
    Purpose
    this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    with 6 Y in 3 ITAMs mutated to L
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153546-plasmid-map-sequence-id-305909

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  20. pET-30a(+)-N-SH2

    Plasmid
    #111274
    Purpose
    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Use
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111274-plasmid-map-sequence-id-215709

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Showing: 1281 - 1300 of 1648 results