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pET28_Strep-Psc-pScaC

Plasmid

#228834

Purpose

E. coli expression of the ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

Strep

Expression

Bacterial

Mutation

aa 33-223

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_pScaC(50-159)

Plasmid

#228836

Purpose

E. coli expression of a truncated ScaC passenger domain (aa 50-159)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

Strep

Expression

Bacterial

Mutation

aa 50-159

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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Pmo_slit-1

Plasmid

#107648

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

slit-1

Use

RNAi; In situ hybridization

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Pmo_sfrp-1

Plasmid

#107649

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

sfrp-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Pmo_innexin-10

Plasmid

#107645

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

innexin-10

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Pmo_piwi-1

Plasmid

#107640

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

piwi-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Pmo_frz-4

Plasmid

#107650

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

frz-4

Use

RNAi; In situ hybridization

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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T4 Lysozyme L31d-3=L31d WT*

Plasmid

#18256

Depositor

Brian Matthews

Article

Sagermann et al Protein Sci. 2006 May . 15(5):1085-92.

Insert

T4 Lysozyme (e Bacteriophage T4)

Expression

Bacterial

Mutation

insertion of Gly Ile Gly His Leu Leu after I27 WT*

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
APEX2-NLS

Plasmid

#124617

Purpose

proximity biotinylation

Depositor

Alice Ting

Article

Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

Insert

APEX2-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTKEI-Tre-C04

Plasmid

#79754

Purpose

Single fluorescent protein biosensor for trehalose

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Tre-C04

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW LAMP1Sig-mCherry

Plasmid

#239556

Purpose

Expression and lysosomal localization of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR207-Citrine-NLS

Plasmid

#44586

Purpose

Gateway entry vector for Citrine fluorescent protein with a nuclear localization signal

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Tags

NLS (Nuclear Localization Signal)

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW mCherry-P2A-PuroR

Plasmid

#239567

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW COX8ASig-mEmerald

Plasmid

#239557

Purpose

Expression and mitochondrial localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW COX8ASig-mCherry

Plasmid

#239558

Purpose

Expression and mitochondrial localization of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW LAMP1Sig-mEmerald

Plasmid

#239555

Purpose

Expression and lysosomal localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

Plasmid

#32703

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

3x HA tags

Expression

Mammalian

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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BALF5-WT

Plasmid

#192454

Purpose

Expresses wild-type BALF5 (codon-optimized)

Depositor

Bill Sugden

Article

Rosemarie et al mBio. 2023 Feb 28;14(1):e0268622. doi: 1

Insert

BALF5 (WT) (BALF5 Epstein-Barr Virus)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b_pScaC-SNAP_6xHis

Plasmid

#228835

Purpose

E. coli expression of the SNAP-tagged ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

SNAPtag-TEV-6xHis

Expression

Bacterial

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-FKBP-HaloTag

Plasmid

#233350

Purpose

Stable expression of FKBP-Halotag in mammalian cells. FKBP-halotag localizes in the cytosol and binds to the FRB tag in the presence of AP21967 (A/C Heterodimerizer).

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Insert

FKBP

Use

Retroviral

Tags

HaloTag7

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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