Addgene

We narrowed to 31,335 results for: Eng

Showing: 1951 - 1980 of 31335 results

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DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228652

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 4 portion of NS2B protease fused to NS3

Use

Tags

His-Twin Strep-Tev

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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DENV-2 strain Thailand/16681/84 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228650

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 2 for biochemical assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 2 portion of NS2B protease fused to NS3

Use

Tags

His-Twin Strep-Tev

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pIR3DH8K

Plasmid

#165069

Purpose

Knocking out GAL80 using KanMX4 marker

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

gal80Arm1-P-TPI1-KanMX4-gal80Arm2

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pIR3DH8

Plasmid

#165068

Purpose

Knocking out GAL80 using KlURA3 marker

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

gal80Arm1-P-AgTEF1-KlURA3-T-AgTEF1-gal80Arm2

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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siCheck2-6xmiR307_23nt-nearperfect

Plasmid

#41099

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

6xmiR307_23nt-nearperfect

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
siCheck2-8xmiR307_23nt-seedonly

Plasmid

#41102

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

8xmiR307_23nt-seedonly

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-8xmiR307_21nt-seedonly

Plasmid

#41103

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

8xmiR307_21nt-seedonly

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-4xmiR307_21nt-seedonly

Plasmid

#41101

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

4xmiR307_21nt-seedonly

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-4xmiR307_23nt-seedonly

Plasmid

#41100

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

4xmiR307_23nt-seedonly

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-6xmiR307_21nt-nearperfect

Plasmid

#41098

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

6xmiR307_21nt-nearperfect

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-taranismutant3'UTR

Plasmid

#41107

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

taranismutant3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-taranis3'UTR

Plasmid

#41106

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

taranis3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-Gkmutant3'UTR

Plasmid

#41105

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Gk3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-Gk3'UTR

Plasmid

#41104

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Gk3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pHTC-HaloTag_FBXO3:1-471

Plasmid

#211072

Purpose

Mammalian expression of full-length human FBXO3. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXO3:M1-F471 (FBXO3 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_DCAF1:1-1507

Plasmid

#210972

Purpose

Mammalian expression of full-length human DCAF1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTC-HaloTag_DCAF1:1-1507

Plasmid

#210973

Purpose

Mammalian expression of full-length human DCAF1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_CBFA2T3:1-615

Plasmid

#211070

Purpose

Mammalian expression of full-length human CBFA2T3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CBFA2T3:M1-R615 (CBFA2T3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

not annotated isoform

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_GEMIN5:1-1508

Plasmid

#211164

Purpose

Mammalian expression of full-length human GEMIN5. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

Gemin5:M1-M1508 (GEMIN5 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CORO1B:1-489

Plasmid

#211196

Purpose

Mammalian expression of full-length human CORO1B. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1B:M1-A489 (CORO1B Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_AAMP:1-434

Plasmid

#211265

Purpose

Mammalian expression of full-length human AAMP. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

AAMP:M1-R434 (AAMP Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFBD-BirA_RACK1:1-317

Plasmid

#210889

Purpose

Insect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RACK1:M1-R317 (RACK1 Human)

Use

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTN-HaloTag_RAB7A:1-207

Plasmid

#211095

Purpose

Mammalian expression of full-length human RAB7A. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RAB7A:M1-C207 (RAB7A Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTN-HaloTag_FBXO3:1-471

Plasmid

#211071

Purpose

Mammalian expression of full-length human FBXO3. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXO3:M1-F471 (FBXO3 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBiT3.1-C_DNAI1:1-699

Plasmid

#211275

Purpose

Mammalian expression of full-length human DNAI1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DNAI1:M1-T699 (DNAI1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

A8S Y202C P495Q annotated sequence conflicts

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-N_CRBN:1-442

Plasmid

#211066

Purpose

Mammalian expression of full-length human CRBN. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CRBN:M1-L442 (CRBN Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_CORO1A:1-461

Plasmid

#211111

Purpose

Mammalian expression of full-length human CORO1A. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTN-HaloTag_RPA2:1-270

Plasmid

#211013

Purpose

Mammalian expression of full-length human RPA2. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RPA2:M1-E270 (RPA2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNLF1-N_BTRC:1-605

Plasmid

#211059

Purpose

Mammalian expression of full-length human BTRC. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

BTRC:M1-R605 (BTRC Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_CRBN:1-442

Plasmid

#211067

Purpose

Mammalian expression of full-length human CRBN the C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CRBN:M1-L442 (CRBN Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge