Addgene

We narrowed to 12,329 results for: BASE;

Showing: 1301 - 1320 of 12329 results

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nSaCas9 M732 miniABEMax V82G

Plasmid

#135382

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 M732 miniABEMax V82G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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nSaCas9 M741 miniABEMax V82G

Plasmid

#135383

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 M741 miniABEMax V82G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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nSaCas9 N120 miniABEMax V82G

Plasmid

#135384

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 N120 miniABEMax V82G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 ABEMax

Plasmid

#135356

Purpose

Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-ABEMax aa 1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 V82G ABEMax

Plasmid

#135361

Purpose

Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1058 nSpyCas9 V82G ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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miniABEMax V82G nSaCas9

Plasmid

#135365

Purpose

Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax V82G nSaCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Blast

Plasmid

#83480

Purpose

Mammalian expression of Cas9 and sgRNA scaffold

Depositor

Mohan Babu

Insert

Blasticidin S deaminase

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Replaced puromycin N-acetyltransferase on the ori…

Promoter

EF-1α

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pBB542

Plasmid

#27395

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

dnaK, dnaJ, groESL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

grpE, clpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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pBB550

Plasmid

#27396

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

dnaK, dnaJ, GroESL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.puro

Plasmid

#32682

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only

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p324_DG

Plasmid

#234690

Purpose

Golden gate domesticated pET28b with TEV-His6

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

TEV-His6

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p368_DG

Plasmid

#234691

Purpose

Golden gate domesticated pET28b with 3C-His6

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

3C-His6

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p369_DG

Plasmid

#234692

Purpose

Golden gate domesticated pET28b with 3C-His10

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

3C-His10

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p344_DG

Plasmid

#234674

Purpose

Golden gate domesticated pET28b with His6-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His6-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p346_DG

Plasmid

#234676

Purpose

Golden gate domesticated pET28b with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p350_DG

Plasmid

#234680

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-bdNEDD8

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p345_DG

Plasmid

#234675

Purpose

Golden gate domesticated pET28b with His10-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p347_DG

Plasmid

#234677

Purpose

Golden gate domesticated pET28b with His10-Thrombin

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-Thrombin

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.DEST.EGFP

Plasmid

#32684

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge