We narrowed to 3,649 results for: WOL

1. pDONR221 Ub-NLRP1 L1218

   Plasmid

   #166829

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 NLRP1 FIIND (F1079M-L1373)

   Plasmid

   #166831

   Purpose

   Gateway pDONR vector containing NLRP1 FIIND (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. dTAG NLRP1 P1214R

   Plasmid

   #166826

   Purpose

   dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P1214R

   Promoter

   hPGK

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-f1(-)-TOMM20-dTomato

   Plasmid

   #115922

   Purpose

   donor to insert dTomato at the TOMM20 locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   TOMM20-dTomato
   

   Use

   Donor template

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-f1(+)-GAPDH-IRES-dTomato

   Plasmid

   #115912

   Purpose

   donor to insert IRES-dTomato at the GAPDH locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   IRES-dTomato
   

   Use

   Donor template

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R495E

   Plasmid

   #164016

   Purpose

   Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R495E

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 6B CARD8 UPA-CARD-mCherry Y527A

   Plasmid

   #164018

   Purpose

   Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Y527A

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB.His.MBP.3C CARD8 CARD A452C

   Plasmid

   #164023

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (A452C mutation for cysteine labelling applications)

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   A452C

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-FnCas12a-TLR-MCV1-gRNA

   Plasmid

   #117412

   Purpose

   FnCas12a-gRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   FnCas12a gRNA targeting TLR 2.0
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTone-gata2aECE-EGFP

    Plasmid

    #132974

    Purpose

    gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Inserts

    eGFP
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
    

    Use

    Unspecified; Transposon-mediated

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-LAP-BUB1

    Plasmid

    #115903

    Purpose

    Used for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

    Insert

    BUB1 (BUB1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK-ATF3

    Plasmid

    #26115

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiCRISPRv4

    Plasmid

    #221432

    Purpose

    Lentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJC6-3XHA-TMEM192

    Plasmid

    #104434

    Purpose

    Expresses 3XHA-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK-ATF4

    Plasmid

    #26114

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-PTK7-VSV

    Plasmid

    #65250

    Purpose

    Expression of VSV tagged PTK7 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

    Insert

    PTK7 (PTK7 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Braf

    Plasmid

    #40775

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    Braf (BRAF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-FLAG-SLC38A9.1

    Plasmid

    #71855

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-myc3-HDM2

    Plasmid

    #20935

    Depositor

    Yue Xiong

    Article

    Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

    Insert

    Mdm2 p53 binding protein homolog (MDM2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-mDyn3 pcDNA3

    Plasmid

    #36265

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 3 (Dnm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only