We narrowed to 20,032 results for: crispr plasmids

Showing: 1301 - 1320 of 20032 results

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PX458_HHEX_2

Plasmid

#72355

Purpose

Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

HHEX (HHEX Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pJAT30

Plasmid

#204294

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT30

Plasmid

#204294

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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AIO-Puro

Plasmid

#74630

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Puromycin resistant marker

Expression

Mammalian

Promoter

Cbh

Available Since

May 6, 2016

Availability

Academic Institutions and Nonprofits only

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AIO-Puro

Plasmid

#74630

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Puromycin resistant marker

Expression

Mammalian

Promoter

Cbh

Available Since

May 6, 2016

Availability

Academic Institutions and Nonprofits only

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pORFE1001

Plasmid

#112079

Purpose

Overexpression of the AtCAS9 in plant under 2x35S promoter

Depositor

Christian Chevalier

Insert

AtCAS9

Use

CRISPR

Expression

Plant

Mutation

Plant codon optimized Cas9 S. pyogenes

Promoter

2x35S

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pORFE1001

Plasmid

#112079

Purpose

Overexpression of the AtCAS9 in plant under 2x35S promoter

Depositor

Christian Chevalier

Insert

AtCAS9

Use

CRISPR

Expression

Plant

Mutation

Plant codon optimized Cas9 S. pyogenes

Promoter

2x35S

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pORFE0002

Plasmid

#112071

Purpose

AtCAS9 nickase D10A module CDS1

Depositor

Christian Chevalier

Insert

AtCAS9 nickase D10A

Use

CRISPR

Expression

Plant

Mutation

Plant codon optimized nCas9 D10A S. pyogenes

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pORFE0002

Plasmid

#112071

Purpose

AtCAS9 nickase D10A module CDS1

Depositor

Christian Chevalier

Insert

AtCAS9 nickase D10A

Use

CRISPR

Expression

Plant

Mutation

Plant codon optimized nCas9 D10A S. pyogenes

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pORFE0003

Plasmid

#112072

Purpose

AtDeadCAS9 D10A H840A module CDS1

Depositor

Christian Chevalier

Insert

AtDeadCAS9 D10A H840A

Use

CRISPR

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pORFE0003

Plasmid

#112072

Purpose

AtDeadCAS9 D10A H840A module CDS1

Depositor

Christian Chevalier

Insert

AtDeadCAS9 D10A H840A

Use

CRISPR

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pC004 - PFS library in Beta-lactamase gene Target Plasmid

Pooled Library

#79153

Purpose

Library of protospacer flanking sites (PFS) inserted at the 5' end of the β-lactamase gene for screening PFS preference for Cas13a/C2c2.

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only
pZFX-donor

Plasmid

#112366

Purpose

CRISPR donor plasmid to tag human transcription factor ZFX with GFP

Depositor

Kevin White

Insert

ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)

Use

CRISPR

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pZFX-donor

Plasmid

#112366

Purpose

CRISPR donor plasmid to tag human transcription factor ZFX with GFP

Depositor

Kevin White

Insert

ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)

Use

CRISPR

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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p-dCas9-VP64-Hygro

Plasmid

#104405

Purpose

transient expression of dCas9-VP64 fusion protein

Depositor

Stephan Beck

Insert

VP64

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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p-dCas9-VP64-Hygro

Plasmid

#104405

Purpose

transient expression of dCas9-VP64 fusion protein

Depositor

Stephan Beck

Insert

VP64

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSBtet-RB-PE2

Plasmid

#173903

Purpose

SB-transposon with inducible expression of SpCas9 PE2

Depositor

Ronald Cohn

Insert

PE2

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSBtet-RB-PE2

Plasmid

#173903

Purpose

SB-transposon with inducible expression of SpCas9 PE2

Depositor

Ronald Cohn

Insert

PE2

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pZBTB5-donor

Plasmid

#112303

Purpose

CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP

Depositor

Kevin White

Insert

ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)

Use

CRISPR

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pZBTB5-donor

Plasmid

#112303

Purpose

CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP

Depositor

Kevin White

Insert

ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)

Use

CRISPR

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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