We narrowed to 1,687 results for: plasmid for e coli

1. pSCL.180

   Plasmid

   #185001

   Purpose

   Express -Eco1 AAVS1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   AAVS1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL.178

   Plasmid

   #184999

   Purpose

   Express -Eco1 FANCF editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   FANCF donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCL.176

   Plasmid

   #184997

   Purpose

   Express -Eco1 RNF2 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   RNF2 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCL.179

   Plasmid

   #185000

   Purpose

   Express -Eco1 HEK4 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   HEK4 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIM1773-PRHIII-sfGFP-pNW33N

   Plasmid

   #52217

   Purpose

   Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli

   Depositor

   Ichiro Matsumura

   Article

   Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

   Insert

   PRHIII-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PRHIII

   Available since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRMCD28

   Plasmid

   #167556

   Purpose

   Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

   Depositor

   Renato Morona

   Article

   Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

   Type

   Empty backbone

   Use

   Tags

   PhoA'

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIB184-Km

   Plasmid

   #90195

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

   Depositor

   Indranil Biswas

   Article

   Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcrRNA.con

   Plasmid

   #61285

   Purpose

   Constitutive repeat-only E. coli CRISPR array

   Depositor

   Chase Beisel

   Article

   Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.26-SS-358

   Plasmid

   #68790

   Purpose

   ZF9 Op x6 -- NTR

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF9 Op x6
   Nitroreductase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRMCD70

    Plasmid

    #167557

    Purpose

    Plasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mapping

    Depositor

    Renato Morona

    Article

    Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

    Type

    Empty backbone

    Use

    Tags

    LacZ'

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

    Plasmid

    #169191

    Purpose

    To express nsp10-nsp16 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

    Insert

    14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGST2-GST-TEV-NDP52

    Plasmid

    #187828

    Purpose

    Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

    Plasmid

    #196703

    Purpose

    Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 D210N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D210N, catalytically inactive

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CGBE1 (pRZ3885)

    Plasmid

    #140252

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas-CmR(+)

    Plasmid

    #113633

    Purpose

    Chloramphenicol resistant derivative of pCas plasmid

    Depositor

    Olivier Lichtarge

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    Chloramphenicol resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Pcat

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-hSynI-rtTAV16

    Plasmid

    #102367

    Purpose

    AAV vector to drive TET-ON activator expression from human synapsin I promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

    Insert

    rtTAV16
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    rtTA variant reported in Gene Therapy (2006) 13, …

    Promoter

    human synapsin I promoter

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CGBE1-VRQR (pBM1192)

    Plasmid

    #140254

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge