We narrowed to 1,719 results for: plasmid for e coli

1. pRSFDUET-1_VNp-anti-Muc1 mAb

   Plasmid

   #214954

   Purpose

   Bacterial expression of anti-Muc1 (human) mAb light and heavy chain VNp fusions.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-Muc1 mAb heavy chain (MUC1 )
   VNp-anti-Muc1 mAb light chain (MUC1 )
   

   Use

   Tags

   6xHis and VNp

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRE4-Bpa

   Plasmid

   #155342

   Purpose

   Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

   Depositor

   Irene Coin

   Article

   Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

   Insert

   humanized EBpaRS/BstYam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/U6

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-eDHFR

   Plasmid

   #107266

   Purpose

   mCherry-eDHFR in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   mCherry-eDHFR (dhfr E.coli)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXD69m2-AphA

   Plasmid

   #191247

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-22b(+) pCopA sfgfp

   Plasmid

   #226374

   Purpose

   Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

   Depositor

   Urartu Seker

   Article

   Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

   Insert

   sfGFP
   

   Use

   Tags

   6x HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   pCopA

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-22b(+) pCopA RBS30 sfgfp

   Plasmid

   #226371

   Purpose

   Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

   Depositor

   Urartu Seker

   Article

   Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

   Insert

   sfGFP
   

   Use

   Tags

   6x HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   copA promoter (pCopA) and RBS30 regulation

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJJGL001

   Plasmid

   #180605

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   10x His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJGL002

   Plasmid

   #180606

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   PlmCasX
   

   Use

   CRISPR

   Tags

   10xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJGL003

   Plasmid

   #180607

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   10x His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Prham-M.EcoRI-p15A-aadA

    Plasmid

    #149341

    Purpose

    expresses M.EcoRI DNA methyltransferase in E. coli

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

    Insert

    M.EcoRI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCN34e-yfp

    Plasmid

    #227641

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCN38-yfp

    Plasmid

    #227637

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIA1414

    Plasmid

    #172402

    Purpose

    Expression of SARS-COV-2 Nsp9 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIA1400

    Plasmid

    #172519

    Purpose

    Expression of SARS-COV-2 Nsp12 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIA1364

    Plasmid

    #172401

    Purpose

    Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIA1405

    Plasmid

    #172404

    Purpose

    Expression of SARS-COV-2 Nsp12 D218A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D218A

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIA1420

    Plasmid

    #172406

    Purpose

    Expression of SARS-COV-2 Nsp12 R116A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    R116A

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIA1421

    Plasmid

    #172407

    Purpose

    Expression of SARS-COV-2 Nsp12 K50A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIA1402

    Plasmid

    #172403

    Purpose

    Expression of SARS-COV-2 Nsp12 Y129A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Y129A

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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