We narrowed to 1,714 results for: plasmid for e coli

1. pET-22b(+) pCopA RBS30 sfgfp

   Plasmid

   #226371

   Purpose

   Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

   Depositor

   Urartu Seker

   Article

   Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

   Insert

   sfGFP
   

   Use

   Tags

   6x HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   copA promoter (pCopA) and RBS30 regulation

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJGL001

   Plasmid

   #180605

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   10x His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJJGL002

   Plasmid

   #180606

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   PlmCasX
   

   Use

   CRISPR

   Tags

   10xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJJGL003

   Plasmid

   #180607

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   10x His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUK21

   Plasmid

   #49788

   Purpose

   E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lacZ

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Prham-M.EcoRI-p15A-aadA

   Plasmid

   #149341

   Purpose

   expresses M.EcoRI DNA methyltransferase in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

   Insert

   M.EcoRI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCN38-yfp

   Plasmid

   #227637

   Purpose

   Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIA1414

   Plasmid

   #172402

   Purpose

   Expression of SARS-COV-2 Nsp9 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp9 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIA1400

   Plasmid

   #172519

   Purpose

   Expression of SARS-COV-2 Nsp12 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIA1364

    Plasmid

    #172401

    Purpose

    Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIA1405

    Plasmid

    #172404

    Purpose

    Expression of SARS-COV-2 Nsp12 D218A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D218A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIA1420

    Plasmid

    #172406

    Purpose

    Expression of SARS-COV-2 Nsp12 R116A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    R116A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIA1421

    Plasmid

    #172407

    Purpose

    Expression of SARS-COV-2 Nsp12 K50A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIA1402

    Plasmid

    #172403

    Purpose

    Expression of SARS-COV-2 Nsp12 Y129A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Y129A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIA1408

    Plasmid

    #172405

    Purpose

    Expression of SARS-COV-2 Nsp12 D760A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D760A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCN34e-yfp

    Plasmid

    #227641

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-RNaseHI-NES-EGFP

    Plasmid

    #196701

    Purpose

    Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    bacterial RNaseHI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_Thy1StTA

    Plasmid

    #97411

    Purpose

    TET driver for amplified expression in cortical neurons

    Depositor

    Tetsuo Yamamori

    Article

    Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse thy1PSs

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKD068

    Plasmid

    #136471

    Purpose

    SSB-GFP IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-GFP (ssb E. coli)
    

    Use

    Tags

    superfolder GFP

    Expression

    Bacterial

    Mutation

    superfolder GFP inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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