We narrowed to 10,022 results for: transfer

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pCSmEn25EERT(#232)

Plasmid

#184081

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

6his-myc-chkEn2(5E)-ERT2

Use

Synthetic Biology

Tags

6his-Myc

Expression

Mammalian

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

sCMV+SP6

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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Syn61Δ3(ev5) ΔrecA (ev1)

Bacterial Strain

#189857

Purpose

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

Depositor

George Church

Article

Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

Bacterial Resistance

Streptomycin

Species

Synthetic

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-tdTomato (codon diversified)

Plasmid

#59462

Purpose

AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter

Depositor

Edward Boyden

Has Service

AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

tdTomato

Use

AAV

Expression

Mammalian

Mutation

codon diversified

Promoter

CAG

Available Since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

Plasmid

#159902

Purpose

Mutagenesis of Slc6a3

Depositor

Larry Zweifel

Insert

Slc6a3 (Slc6a3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-mCherry

Plasmid

#50459

Purpose

Double floxed mCherry under the control of human synapsin promoter

Depositor

Bryan Roth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

human Synapsin 1

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-EGFP

Plasmid

#50457

Purpose

Double floxed EGFP under the control of human synapsin promoter

Depositor

Bryan Roth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP

Use

AAV

Tags

N/A

Promoter

human Synapsin 1

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-phSyn-flex-SypEGFP

Plasmid

#153203

Purpose

Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Lynette Lim

Insert

synaptophysin-EGFP

Use

AAV

Promoter

rat synapsin

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

Plasmid

#192552

Purpose

Expresses nuclear localized tdTomato under CAG promoter

Depositor

Guoping Feng

Has Service

AAV PHP.eB and AAV9

Insert

tdTomato

Use

AAV

Tags

NLS

Mutation

deleted E273 and G274 compared to FPbase ID: PGG5S

Promoter

CAG

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-lck-jGCaMP8s

Plasmid

#176761

Purpose

AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter

Depositor

Loren Looger

Has Service

AAV1 and AAV9

Insert

lck-jGCaMP8s

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-jGCaMP8s-WPRE

Plasmid

#176752

Purpose

AAV-mediated expression of GCaMP8s under the CamKIIa promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

CamKIIalpha

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-sCAG-mBaoJin-oPRE

Plasmid

#218684

Purpose

AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

Depositor

Bart Nieuwenhuis

Insert

mBaoJin

Use

AAV

Expression

Mammalian

Promoter

Short variant of the CAG promoter

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-hM3D(Gq)-mCherry

Plasmid

#50478

Purpose

Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter

Depositor

Bryan Roth

Has Service

AAV5

Insert

hM3D(Gq)-mCherry

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.PI.FLPo.rBG

Plasmid

#236288

Purpose

AAV vector expressing FLPo from TBG promoter

Depositor

Melina Fan, Addgene Research Program

Has Service

AAV8

Insert

FLPo

Use

AAV

Promoter

TBG

Available Since

May 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-CamKIIa-jGCaMP8f-WPRE

Plasmid

#176750

Purpose

AAV-mediated expression of GCaMP8f under the CamKIIa promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

CamKIIalpha

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn GFP-Cre

Plasmid

#175381

Purpose

expresses GFP-Cre under the control of a human synapsin promoter

Depositor

Jason Aoto

Insert

GFP-Cre recombinase

Use

AAV

Tags

Cre is fused to GFP. GFP is fused to additional n…

Promoter

human synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-mDlx-jGCaMP8s-WPRE

Plasmid

#176755

Purpose

jGCaMP8s expression in GABA-ergic interneurons under the control of mDlx enhancer

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

mDlx

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-hM4D(Gi)-mCherry

Plasmid

#50479

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

Depositor

Bryan Roth

Has Service

AAV5

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-CAG-hM4Di-P2A-dTomato

Plasmid

#202037

Purpose

AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

Depositor

Yoav Ben-Simon, Peter Jonas

Insert

hM4Di

Use

AAV

Tags

dTomato

Promoter

CAG

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-jGCaMP8m-WPRE

Plasmid

#176751

Purpose

AAV-mediated expression of GCaMP8m under the CamKIIa promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

CamKIIalpha

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-CAG-hM3Dq-P2A-dTomato

Plasmid

#202036

Purpose

AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato

Depositor

Yoav Ben-Simon, Peter Jonas

Insert

hM3Dq

Use

AAV

Tags

dTomato

Promoter

CAG

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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