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Plasmid#31078DepositorInsertMature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Ami…PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPEE17
Plasmid#128361PurposeDetecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.DepositorInsertAcetamidase (amdS)
UseUnspecifiedTagsExpressionMutationPromoterTEF1Available sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
BcLOV-mCh-SOS
Plasmid#208279PurposeExpresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDH3
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
UseTagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …PromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLLX13
Plasmid#79825PurposeYeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vectorDepositorTypeEmpty backboneUseYeast and bacteria shuttle vector, contains origi…TagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9GFP-shP53
Plasmid#102906PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.DepositorInsertdCas9GFP-shP53
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dppa1-T2A-H2B-Halo donor
Plasmid#113118PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequenceDepositorInsertDppa1 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-ChBP
Plasmid#108879PurposeExpression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c001
Plasmid#175448PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.DepositorInsertCD44 (CD44 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY134
Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only