We narrowed to 9,142 results for: App

1. pAAV-hsyn-dMASS_LF_WPRE

   Plasmid

   #209766

   Purpose

   AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   dMASS_LF
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_hsyn_HA-Flag-uMASS_1_WPRE

   Plasmid

   #209760

   Purpose

   AAV virus production for neuronal expression of uMASS_1 under hSyn promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   uMASS_1
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-HA_aa 1-542 POLK

   Plasmid

   #201585

   Purpose

   Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   Polymerase kappa (POLK Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-542 of DNA polymerase kap…

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

   Plasmid

   #201533

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE_RBS3_CB2
   

   Use

   Synthetic Biology

   Mutation

   rhaR L128+1bp (frameshift), ilvA G455C

   Promoter

   PrhaBAD (rhamnose)

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

   Plasmid

   #201535

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE_RBS3_CB4
   

   Use

   Synthetic Biology

   Mutation

   rhaS ΔM1-H5, PrhaSR Δ179bp

   Promoter

   PrhaBAD (rhamnose)

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p415 δCLD2α

   Plasmid

   #73874

   Purpose

   δCLD2α - His tagged (μHD deleted)

   Depositor

   Blanche Schwappach

   Article

   Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Arcn1/ δCLD2α (ARCN1 Bovine)
   

   Tags

   His

   Expression

   Yeast

   Promoter

   Met25

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNC2

   Plasmid

   #72438

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg

   Promoter

   T7 and n/a

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK516

   Plasmid

   #71578

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamate-435 to glutamine

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK524

   Plasmid

   #71580

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes asparagine-347 to alanine

   Promoter

   T7

   Available Since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK513

    Plasmid

    #71576

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-294 to alanine

    Promoter

    T7

    Available Since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK564

    Plasmid

    #71581

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes serine-71 to alanine

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK517

    Plasmid

    #71579

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to aspartate

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK514

    Plasmid

    #71577

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamine-435 to alanine

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK512

    Plasmid

    #71575

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes arginine-228 to glutamate

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGS1122

    Plasmid

    #63790

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eSapphire-Twin-Strep tag followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    eSapphire-Twin-Strep tag

    Promoter

    none

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK048

    Plasmid

    #75230

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and n/a

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXB1-pA-M.EcoGII

    Plasmid

    #192873

    Purpose

    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

    Depositor

    Chong Tang

    Article

    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

    Insert

    pA-M.EcoGII
    

    Tags

    3X Flag Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. betaTrCP mVenus reporter

    Plasmid

    #192458

    Purpose

    Fluorescent reporter for beta-TrCP activity in human cells

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CDC25B (CDC25B Human)
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338

    Promoter

    EF1alpha

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEMS-Halo7Tag-hFis

    Plasmid

    #111136

    Purpose

    hFis with selflabeling tag, e.g. for fluorescence microscopy

    Depositor

    Karin Busch

    Article

    Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

    Insert

    human fission factor1 (FIS1 Human)
    

    Tags

    Halo7-tag

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRB133

    Plasmid

    #180176

    Purpose

    GluN2A in pCDNA3.1

    Depositor

    Richard Bergeron

    Article

    Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

    Insert

    GluN2A (Grin2a Rat)
    

    Expression

    Mammalian

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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