We narrowed to 3,866 results for: D10

1. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRH2502

   Plasmid

   #84379

   Purpose

   Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   dcas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Aspartate at position 10 changed to Alanine, Hist…

   Promoter

   uv15tetO

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCM167-Venus-VF-hADAM10(aa220-654)

   Plasmid

   #189560

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 220-654

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCM167-Venus-VF-hADAM10(aa1-459)

   Plasmid

   #189559

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 1-459

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ262m3

   Plasmid

   #149556

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Promoter

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2262

   Plasmid

   #70703

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A & H557A in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET51b-His-TEV-HaloTag7_dead_mt

   Plasmid

   #167267

   Purpose

   Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HaloTag7
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   D106A

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP2279

   Plasmid

   #70706

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A and H557A in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ256

    Plasmid

    #124312

    Purpose

    Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Insert

    pcoCas9D10A-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCM167.2-Venus-VF-hADAM10(aa455-550)

    Plasmid

    #189556

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 455-550

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSCM167-Venus-VF-hADAM10(aa220-459)

    Plasmid

    #189555

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 220-459

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCM167.2-Venus-VF-hADAM10(aa551-654)

    Plasmid

    #189557

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa551-654

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid

    #189562

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    contains aa 1-213

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSCM167-Venus-VF-hADAM10(aa455-654)

    Plasmid

    #189561

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 455-654

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUHG10.3-TetO-Rs1

    Plasmid

    #16313

    Depositor

    Bruce Conklin

    Article

    Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

    Insert

    h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Added FLAG tag; Changed Aspartic Acid 100 to Alan…

    Promoter

    Available since

    Jan. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ255

    Plasmid

    #124310

    Purpose

    Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Insert

    rAPOBEC1-pcoCas9D10A-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSCM167.2-Venus-VF-hADAM10(aa1-654)

    Plasmid

    #189558

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain through cysteine rich domain--amino acids 1-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa1-654

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM1160c2

    Plasmid

    #159724

    Purpose

    Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Use

    Tags

    Gal4DBD and MPGIL-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM1674

    Plasmid

    #159726

    Purpose

    Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Use

    Tags

    Gal4DBD and MPTLV-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge