We narrowed to 18,031 results for: Mut

1. LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP

   Plasmid

   #194934

   Purpose

   LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. -962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic

   Plasmid

   #32733

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 promoter (CCND1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   -75 TCF(1) site CTTTGAT deletion

   Promoter

   -962 CCND1

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

   Plasmid

   #197892

   Purpose

   Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   hM4D/2A/GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   EF1a

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTYB3-human PUM1-HD MUT3-2 (C935S-Q939E)-intein-CBD

   Plasmid

   #73302

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   C935S-Q939E

   Promoter

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

5. -962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic

   Plasmid

   #32736

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 Promoter (CCND1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

   Promoter

   -962 CCND1

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

6. -962 human cyclin D1 promoter EtsA site mutant pGL3Basic

   Plasmid

   #32729

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 promoter (CCND1 Human)
   

   Use

   Luciferase; Luciferase reporter gene

   Tags

   Expression

   Mutation

   -822 EtsA site AGGAA deletion

   Promoter

   -962 CCND1 promoter

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126778

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R691A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. -962 human cyclin D1 promoter EtsB site mutant pGL3Basic

   Plasmid

   #32730

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   -778 EtsB binding site AGGAA deletion

   Promoter

   -962 CCND1

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

9. -962 human cyclin D1 promoter EtsC site mutant pGL3Basic

   Plasmid

   #32731

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 promoter (CCND1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   -424 EtsC site TTCCT deletion

   Promoter

   -962 CCND1 promoter

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

10. -962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic

    Plasmid

    #32734

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…

    Promoter

    -962 CCND1

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc

    Plasmid

    #138367

    Purpose

    Lentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161K human enhancer, with ETS binding sites mutated, driving firefly enhancer and TK driving refills luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126777

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-CMV-Cdc42 3'UTR mutant (position 577-599)

    Plasmid

    #51391

    Depositor

    Narry Kim

    Article

    Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

    Insert

    NM_001039802.1 (CDC42 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    miR-29 binding site mutant (position 577-599)

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

14. -962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

    Plasmid

    #32735

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 Promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

    Promoter

    -962 CCND1

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. human EB1-GFP mutagenized to remove terminal 7 aa (QEEQEEY) (JB162))

    Plasmid

    #39308

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-terminal 7 aa (QEEQEEY) removed

    Promoter

    CMV

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

16. Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874)

    Plasmid

    #239353

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(WT)-mascRNA(mut 8356-8370) reporter.

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    MALAT1 ENE(WT)-mascRNA(mut 8356-8370)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MALAT1 ENE(WT)-mascRNA(mut 8356-8370: ctacgaccacc…

    Promoter

    Available Since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUHD 10-3-human CDK11 p110B D543→N mut. cat.-FLAG

    Plasmid

    #240139

    Purpose

    Express CDK11 p110B D543N mutant

    Depositor

    Martine Roussel, Jill Lahti, Vincent Kidd

    Article

    Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

    Insert

    CDK11 p110 (CDK11A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D543N

    Promoter

    TRE, CMV

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

    Plasmid

    #229742

    Purpose

    Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

    Depositor

    Shawn Ferguson

    Article

    Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    LIR1 mutant WEVL -> AEVA

    Promoter

    EF1A

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

    Plasmid

    #229743

    Purpose

    Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

    Depositor

    Shawn Ferguson

    Article

    Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    LIR2 mutant WTFI -> ATFA

    Promoter

    EF1A

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3puro-mGFP-FXR1-N1-KH12mut (T236D H237D K299D N300D)

    Plasmid

    #225454

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D K299D N300D)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    T236D H237D K299D N300D

    Promoter

    CMV

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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