We narrowed to 16,118 results for: Por

1. pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500

   Plasmid

   #92227

   Purpose

   Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   deGFP Y35TAG D193TAG
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residues Y35 D193 were mutated into TAG (Amber) s…

   Promoter

   p70a

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-C1 R62D actin-3XNLS P2A mCherry

   Plasmid

   #58477

   Purpose

   Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed Arginine 62 to Aspartic Acid

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-bARGSer-AxeTxe

   Plasmid

   #192473

   Purpose

   Second-generation bacterial acoustic reporter gene derived from Serratia

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Inserts

   bARGSer
   AxeTxe
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   the gene Ser39006_001280 was deleted from the clu…

   Promoter

   Native promoter from Enterococcus faecium and pBAD

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-C1 actin-3XNLS P2A mCherry

   Plasmid

   #58475

   Purpose

   Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1-MCU-Flag-TetOFF

   Plasmid

   #185657

   Purpose

   Tet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporter

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   pTight TRE

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

   Plasmid

   #135814

   Purpose

   Fluorescent reporter for knock-in of TdTomato into the TH locus

   Depositor

   Lee Rubin, Tim Ahfeldt

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
   

   Use

   Tags

   p2a-TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP

   Plasmid

   #47633

   Purpose

   An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   hChR2 (H134R)
   

   Use

   AAV and Cre/Lox

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   EF1a

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-MCU-mCerulean

   Plasmid

   #185809

   Purpose

   Human mitochondrial calcium uniporter with C-terminus mCerulean tag

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSin-EF2-DMD53-GFP

   Plasmid

   #138353

   Purpose

   Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences

   Depositor

   Baisong Lu

   Article

   Lyu et al PLoS One. 2020 Sep 24;15(9):e0239468. do

   Insert

   DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   EF2

   Available since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. N229A (EcQ) MetNIQ in pBAD

    Plasmid

    #118257

    Purpose

    N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (N229A) (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 229 to Alanine (Ala) and…

    Promoter

    None and araBAD Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

    Plasmid

    #118581

    Purpose

    N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 229 to Alanine (Ala) and…

    Promoter

    None and araBAD Promotoer

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. N295A (EcN) MetNIQ in pBAD

    Plasmid

    #118256

    Purpose

    N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 295 to Alanine (Ala)

    Promoter

    None and araBAD Promotoer

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid

    #118260

    Purpose

    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCRbluntII-pHES7-STOPgRNA

    Plasmid

    #204349

    Purpose

    gRNA to target pig HES7 stop codon

    Depositor

    Li-Fang Chu

    Article

    Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

    Insert

    Pig HES7-STOP-gRNA (HES7 Sus domesticus (pig))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mGFP-hNET-A457P

    Plasmid

    #193365

    Purpose

    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

    Depositor

    Harald Sitte

    Article

    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

    Insert

    human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
    

    Use

    Tags

    monomeric GFP (mGFP)

    Expression

    Mammalian

    Mutation

    A457P (GCC to CCC)

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mGFP-hNET-R121A/K334A/R440A

    Plasmid

    #193366

    Purpose

    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

    Depositor

    Harald Sitte

    Article

    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

    Insert

    human Norepinephrine Transporter (SLC6A2 Human)
    

    Use

    Tags

    monomeric GFP (mGFP)

    Expression

    Mammalian

    Mutation

    R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. N295A (EcN), M163A (EcI) MetNIQ in pBAD

    Plasmid

    #118261

    Purpose

    N295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (M163A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. N295A (EcN), M107A (EcI) MetNIQ in pBAD

    Plasmid

    #118259

    Purpose

    N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (M107A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. N295A (EcN), F103A (EcI) MetNIQ in pBAD

    Plasmid

    #118258

    Purpose

    N295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (F103A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GoldenPiCS Kit

    Plasmid Kit

    #1000000133

    Purpose

    GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites

    Depositor

    Brigitte Gasser

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only