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We narrowed to 5,610 results for: SPI

Showing: 1321 - 1340 of 5610 results

  11. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid
    #224474
    Purpose
    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    EFS promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    224474-plasmid-map-sequence-id-447208

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  13. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

    Plasmid
    #224505
    Purpose
    Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    LexA-mODC-LexOP-35SCaMV
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    224505-plasmid-map-sequence-id-447028

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  16. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

    Plasmid
    #224512
    Purpose
    A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    3xHA-BirA-T2A-mCherry-Caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    224512-plasmid-map-sequence-id-446939

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  19. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182682
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182682-plasmid-map-sequence-id-363082

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  20. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

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Showing: 1321 - 1340 of 5610 results