We narrowed to 5,317 results for: golden gate

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pPOM001_P1_ConL6

Plasmid

#216426

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L6

Use

Synthetic Biology

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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L0-T6 Empty target Bxb1

Plasmid

#195889

Purpose

Level 0 Empty Bxb1 target: attP_Bxb1-BpiI-attB_Bxb1 with golden gate linker in pBLUNT plasmid.

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Empty Bxb1 Target

Use

Synthetic Biology

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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LI D-E TurboID

Plasmid

#209386

Purpose

Golden Gate LI D-E module

Depositor

Rosa Lozano-Durán

Publication

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Cloning vector

Mutation

TurboID gene sequence C249A

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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LI D-E TurboID-GFP

Plasmid

#209387

Purpose

Golden Gate LI D-E module

Depositor

Rosa Lozano-Durán

Publication

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Cloning vector

Mutation

TurboID gene sequence C249A

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LI B-C TurboID

Plasmid

#209385

Purpose

Golden Gate LI B-C module

Depositor

Rosa Lozano-Durán

Publication

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Cloning vector

Mutation

TurboID gene sequence C249A

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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L0-T1 Target PhiC31

Plasmid

#195890

Purpose

Level 0 PhiC31 target: attP_PhiC31-pUBQ10-attB_PhiC31 with golden gate linker in pBLUNT plasmid.

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 Target

Use

Synthetic Biology

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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L0-I42 Tp901 GG1-GG4

Plasmid

#195907

Purpose

Level 0 Tp901 integrase with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Tp901 integrae

Use

Synthetic Biology

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I14 DST GG4-GG5

Plasmid

#195896

Purpose

Level 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkers

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

DST

Use

Synthetic Biology

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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GGDestX2-Amp

Plasmid

#157654

Purpose

Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

Depositor

Karmella Haynes

Publication

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6, CBh

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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EC71102

Plasmid

#154065

Purpose

Level 0 Golden Gate vector; Unmodifed CRE

Depositor

Cristobal Uauy

Publication

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE

Use

Synthetic Biology; Standard cloning vector used b…

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pNAM-B1

Plasmid

#154056

Purpose

Level 0 Golden Gate vector, contains domesticated NAM-B1

Depositor

Cristobal Uauy

Publication

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

TaNAM-B1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Synonymous mutation at base 3257, in exon 3, to r…

Promoter

N/A

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ131C-RZ-Lb

Plasmid

#134347

Purpose

Golden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoter

Depositor

Yiping Qi

Publication

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFUSC8

Plasmid

#50428

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC7

Plasmid

#50427

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC6

Plasmid

#50426

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC5

Plasmid

#50425

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC4

Plasmid

#50424

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC3

Plasmid

#50423

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC2

Plasmid

#50422

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC10

Plasmid

#50430

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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